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Co-expression of heat shock protein (HSP) 40 and HSP70 in Pinctada martensii response to thermal, low salinity and bacterial challenges
Li, Jun; Zhang, Yuehuan; Liu, Ying; Zhang, Yang; Xiao, Shu; Yu, Ziniu; Yu, ZN (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
2016
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume48Pages:239-243
AbstractHeat shock protein (HSP) 40 proteins are a family of molecular chaperones that bind to HSP70 through their J-domain and regulate the function of HSP70 by stimulating its adenosine triphosphatase activity. In the present study, a HSP40 homolog named PmHSP40 was cloned from the hemocytes of pearl oyster Pinctada martensii using EST and rapid amplification of cDNA ends (RACE) techniques. The full-length cDNA of PmHSP40 was 1251 bp in length, which included a 5' untranslated region (UTR) of 75 bp, an open reading frame (ORF) of a 663 bp, and a 3' UTR of 513 bp. The deduced amino acid sequence of PmHSP40 contains a J domain in the N-terminus. In response to thermal and low salinity stress challenges, the expression of PmHSP40 in hemocytes and the gill were inducible in a time-dependent manner. After bacterial challenge, PmHSP40 transcripts in hemocytes increased and peaked at 6 h post injection. In the gill, PmHSP40 expression increased, similar to expression in hemocytes; however, transcript expression of PmHSP40 was significantly up-regulated at 12 h post injection. Furthermore, the transcripts of PmHSP70 showed similar kinetics as that of PmHSP40, with highest induction during thermal, low salinity stress and bacterial challenges. Altogether these results demonstrate that PmHSP40 is an inducible protein under thermal, low salinity and bacterial challenges, suggesting its involvement in both environmental and biological stresses, and in the innate immunity of the pearl oyster. (C) 2015 Elsevier Ltd. All rights reserved.
Department[Li, Jun; Zhang, Yuehuan; Liu, Ying; Zhang, Yang; Xiao, Shu; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, 164 Wet Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Li, Jun; Zhang, Yuehuan; Liu, Ying; Zhang, Yang; Xiao, Shu; Yu, Ziniu] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China; [Liu, Ying] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China ; LMB
KeywordPinctada Martensii Hsp40 Thermal Stress Low Salinity Bacterial Challenge
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15532
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorYu, ZN (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Li, Jun,Zhang, Yuehuan,Liu, Ying,et al. Co-expression of heat shock protein (HSP) 40 and HSP70 in Pinctada martensii response to thermal, low salinity and bacterial challenges[J]. FISH & SHELLFISH IMMUNOLOGY,2016,48:239-243.
APA Li, Jun.,Zhang, Yuehuan.,Liu, Ying.,Zhang, Yang.,Xiao, Shu.,...&Yu, ZN .(2016).Co-expression of heat shock protein (HSP) 40 and HSP70 in Pinctada martensii response to thermal, low salinity and bacterial challenges.FISH & SHELLFISH IMMUNOLOGY,48,239-243.
MLA Li, Jun,et al."Co-expression of heat shock protein (HSP) 40 and HSP70 in Pinctada martensii response to thermal, low salinity and bacterial challenges".FISH & SHELLFISH IMMUNOLOGY 48(2016):239-243.
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