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Two isoforms of leptin in the White-clouds Mountain minnow (Tanichthys albonubes): Differential regulation by estrogen despite similar response to fasting
Chen, Ting; Chen, Shuang; Ren, Chunhua; Hu, Chaoqun; Tang, Dongsheng; Yan, Aifen; Yan, AF (reprint author), Foshan Univ, Coll Med, Foshan, Peoples R China.
2016
Source PublicationGENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume225Pages:174-184
AbstractLeptin has been well-established as a canonical anorexic peptide hormone in mammals, though much of its function in fish remains obscure. In this study, the cDNAs of two leptin isoforms (leptin-A and leptin-B) were cloned from the liver of a small cyprinid fish, Tanichthys albonubes. The two T. albonubes leptins, sharing low primary amino acid sequence homology with their mammalian counterparts, and between themselves, are highly conserved in three-dimensional protein structures and gene structures. Liver is a major source of leptin mRNA in T. albonubes with leptin-A being the dominant form. The expression of hepatic leptin-A but not leptin-B mRNA in female fish is significantly higher than in male fish. Transcriptional hepatic levels of leptin-A and leptin-B in both male and female fish were demonstrated to increase after long-term fasting (10-25 days) but decline upon re-feeding (3 days). Strikingly, estrogen (E-2) administration induced only leptin-A but not leptin-B hepatic mRNA expression in both male and female fish. Our study here provides the first evidence for differential regulation of two leptins in fish, and sheds new light on the possible origin of leptin in lower vertebrates. (C) 2015 Elsevier Inc. All rights reserved.
Department[Tang, Dongsheng; Yan, Aifen] Foshan Univ, Coll Med, Foshan, Peoples R China; [Chen, Ting; Ren, Chunhua; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou, Guangdong, Peoples R China; [Chen, Ting; Ren, Chunhua; Hu, Chaoqun] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China; [Chen, Shuang] Univ Hong Kong, Dept Anat, Hong Kong, Hong Kong, Peoples R China ; LMB
KeywordLeptin Cyprinid Fish Molecular Cloning Fasting Estrogen Gene Expression
Subject AreaEndocrinology & Metabolism
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/15536
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding AuthorYan, AF (reprint author), Foshan Univ, Coll Med, Foshan, Peoples R China.
Recommended Citation
GB/T 7714
Chen, Ting,Chen, Shuang,Ren, Chunhua,et al. Two isoforms of leptin in the White-clouds Mountain minnow (Tanichthys albonubes): Differential regulation by estrogen despite similar response to fasting[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2016,225:174-184.
APA Chen, Ting.,Chen, Shuang.,Ren, Chunhua.,Hu, Chaoqun.,Tang, Dongsheng.,...&Yan, AF .(2016).Two isoforms of leptin in the White-clouds Mountain minnow (Tanichthys albonubes): Differential regulation by estrogen despite similar response to fasting.GENERAL AND COMPARATIVE ENDOCRINOLOGY,225,174-184.
MLA Chen, Ting,et al."Two isoforms of leptin in the White-clouds Mountain minnow (Tanichthys albonubes): Differential regulation by estrogen despite similar response to fasting".GENERAL AND COMPARATIVE ENDOCRINOLOGY 225(2016):174-184.
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