A rapid and high-throughput microplate spectrophotometric method for field measurement of nitrate in seawater and freshwater
Wu, Jiapeng; Hong, Yiguo; Guan, Fengjie; Wang, Yan; Tan, Yehui; Yue, Weizhong; Wu, Meilin; Bin, Liying; Wang, Jiaping; Wen, Jiali; Hong, YG (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog LTO, Guangzhou 510301, Guangdong, Peoples R China.
2016
发表期刊SCIENTIFIC REPORTS
卷号6页码:20165
摘要The well-known zinc-cadmium reduction method is frequently used for determination of nitrate. However, this method is seldom to be applied on field research of nitrate due to the long time consuming and large sample volume demand. Here, we reported a modified zinc-cadmium reduction method (MZCRM) for measurement of nitrate at natural-abundance level in both seawater and freshwater. The main improvements of MZCRM include using small volume disposable tubes for reaction, a vortex apparatus for shaking to increase reduction rate, and a microplate reader for high-throughput spectrophotometric measurements. Considering salt effect, two salinity sections (5 - 10 psu and 20 - 35 psu) were set up for more accurate determination of nitrate in low and high salinity condition respectively. Under optimized experimental conditions, the reduction rates were stabilized on 72% and 63% on the salinity of 5 and 20 psu respectively. The lowest detection limit for nitrate was 0.5 mu M and was linear up to 100 mu M (RSDs was 4.8%). Environmental samples assay demonstrated that MZCRM was well consistent with conventional zinc-cadmium reduction method. In total, this modified method improved accuracy and efficiency of operations greatly, and would be realized a rapid and highthroughput determination of nitrate in field analysis of nitrate with low cost.
部门归属[Wu, Jiapeng; Hong, Yiguo; Wang, Yan; Yue, Weizhong; Wu, Meilin] Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog LTO, Guangzhou 510301, Guangdong, Peoples R China; [Tan, Yehui] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wu, Jiapeng; Wang, Yan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Guan, Fengjie; Bin, Liying; Wang, Jiaping; Wen, Jiali] Guangdong Univ Technol, Sch Environm Sci & Engn, Guangzhou 510090, Guangdong, Peoples R China ; LTO
学科领域Science & Technology - Other Topics
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/15566
专题热带海洋环境国家重点实验室(LTO)
通讯作者Hong, YG (reprint author), Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog LTO, Guangzhou 510301, Guangdong, Peoples R China.
推荐引用方式
GB/T 7714
Wu, Jiapeng,Hong, Yiguo,Guan, Fengjie,et al. A rapid and high-throughput microplate spectrophotometric method for field measurement of nitrate in seawater and freshwater[J]. SCIENTIFIC REPORTS,2016,6:20165.
APA Wu, Jiapeng.,Hong, Yiguo.,Guan, Fengjie.,Wang, Yan.,Tan, Yehui.,...&Hong, YG .(2016).A rapid and high-throughput microplate spectrophotometric method for field measurement of nitrate in seawater and freshwater.SCIENTIFIC REPORTS,6,20165.
MLA Wu, Jiapeng,et al."A rapid and high-throughput microplate spectrophotometric method for field measurement of nitrate in seawater and freshwater".SCIENTIFIC REPORTS 6(2016):20165.
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