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Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana
Wang, J; Ying, SH; Hu, Y; Feng, MG; mgfeng@zju.edu.cn
2017
Source PublicationAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume101Issue:1Pages:185-195
AbstractMdj1 is a member of the Hsp40 family containing a DnaJ or J domain. Here, we have examined the functions of an Mdj1 orthologue (56.68 kDa) in Beauveria bassiana, a filamentous fungal insect pathogen widely applied in biological control of insect pests. Deletion of mdj1 in B. bassiana resulted in significant growth defects on a variety of complex and minimal media. The Delta mdj1 mutant exhibited not only a drastic reduction (92 %) in aerial conidiation during optimal cultivation but also a remarkable decrease (77 %) in submerged blastospore production. Compared to wild-type, the deletion mutant was significantly more sensitive to the stresses of cell wall perturbation, high osmolarity, oxidation, carbendazim fungicide, several metal ions, and acidic/alkaline pH during conidial germination and/or colony growth. In the mutant, conidial thermotolerance and UV-B resistance decreased by 61 and 25 %, respectively, and virulence to Galleria mellonella larvae was largely attenuated. Transcript levels of many phenotype-related genes were drastically suppressed in the absence of mdj1, accompanied with impaired cell walls and reduced intracellular anti-stress molecules, including superoxide dismutases, catalases, glycerol, trehalose, and mannitol. These data indicate that Mdj1 plays a vital role in normal fungal development and contributes significantly to the biological control potential of B. bassiana against insect pests.
Department[Wang, Jie] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Jie; Ying, Sheng-Hua; Hu, Yue; Feng, Ming-Guang] Zhejiang Univ, Coll Life Sci, Inst Microbiol, Hangzhou 310058, Zhejiang, Peoples R China
KeywordEntomopathogenic Fungi J-domain Proteins Asexual Development Cell Wall Integrity Multiple Stress Tolerance Biological Control Potential
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16210
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authormgfeng@zju.edu.cn
Recommended Citation
GB/T 7714
Wang, J,Ying, SH,Hu, Y,et al. Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(1):185-195.
APA Wang, J,Ying, SH,Hu, Y,Feng, MG,&mgfeng@zju.edu.cn.(2017).Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(1),185-195.
MLA Wang, J,et al."Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.1(2017):185-195.
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