TRAF6 is a critical factor in fish immune response to virus infection
Wei, JG; Zang, SQ; Xu, M; Zheng, QJ; Chen, XL; Qin, QW; qinqw@scsio.ac.cn
2017
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号60页码:6-12
摘要Tumor necrosis factor receptor-associated factor 6 (TRAF6) is one of the key adaptor molecule in Toll-like receptor signal transduction that triggers downstream cascades involved in innate immunity. In our previous study, the molecular characteristics of EtTRAF6 (TRAF6 from Epinephelus tauvina), the tissue distributions, expression patterns after challenging with bacterial and viral pathogens were investigated. Here we identified EtTRAF6 as an important regulator of virus-triggered signaling pathway. Over expression of EtTRAF6-ORF and truncated forms of EtTRAF6, including EtTRAF6-C (delete the MATH domain), EtTRAF6-N (delete the RING domain) and EtTRAF6-MATH, inhibited IFN-beta activity strongly in grouper spleen (GS) cells. Overexpression of EtTRAF6 repressed virus-induced production of type I IFNs. When EtTRAF6 cotransfected with EcIRF3 or EcIRF7, EtTRAF6 inhibited IRF-induced activation of IFN-beta. Over-expressed EtTRAF6 inhibited" the transcription of SGIV genes significantly in GS cells. Although TRAF6 has a role in apoptosis regulation, it is not known if EtTRAF6 has any role in apoptosis regulation. Strikingly, when over-expressed in fathead minnow (FHM) cells, EtTRAF6 protected them from cell death induced by SGIV. Therefore, these results suggest that TRAF6 may play a critical role in their response to SGIV infection, through regulation of a cell death pathway that is common to fish and humans. (C) 2016 Elsevier Ltd. All rights reserved.
部门归属[Wei, Jingguang; Zang, Shaoqing; Chen, Xiuli; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Wei, Jingguang; Zang, Shaoqing; Chen, Xiuli; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China; [Xu, Meng] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China; [Zheng, Qiaojun] Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China
关键词Epinephelus Tauvina Traf6 Interferon Sgiv Apoptosis
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16214
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Wei, JG,Zang, SQ,Xu, M,et al. TRAF6 is a critical factor in fish immune response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2017,60:6-12.
APA Wei, JG.,Zang, SQ.,Xu, M.,Zheng, QJ.,Chen, XL.,...&qinqw@scsio.ac.cn.(2017).TRAF6 is a critical factor in fish immune response to virus infection.FISH & SHELLFISH IMMUNOLOGY,60,6-12.
MLA Wei, JG,et al."TRAF6 is a critical factor in fish immune response to virus infection".FISH & SHELLFISH IMMUNOLOGY 60(2017):6-12.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
TRAF6 is a critical (1044KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。