A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro
Qu, FF; Xiang, ZM; Li, J; Xiao, S; Mao, F; Qin, YP; Zhou, YL; Ma, HT; Yu, ZN; zhimingxiang@scsio.ac.cn; carlzyu@scsio.ac.cn
2017
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号62页码:311-319
摘要Extracellular signal -regulated kinases (ERKs) are a group of highly conserved serine/threonine-specific protein kinases that function as important signaling intermediates in mitogen-activated protein kinase (MAPK) pathways, which are involved in a wide variety of cellular activities, including proliferation, inflammation and cytokine production. However, little is known about the roles of this kinase in mollusk immunity. In this study, we identified a molluscan ERK homolog (ChERK) in the Hong Kong oyster (Crassostrea hongkongensis) and investigated its biological functions. The open reading frame (ORF) of ChERK encoded a polypeptide of 365 amino acids, with a predicted molecular weight of 41.96 kDa and pI of 6.43. The predicted ChERK protein contained typical characteristic motifs of the ERK family, including a dual threonine-glutamate-tyrosine (TEY) phosphorylation motif and an ATRW substrate binding site. Phylogenetic analysis revealed that ChERK belonged to the mollusk cluster and shared a close evolutionary relationship with ERIC from Crassostrea gigas. In addition, quantitative real-time PCR analysis revealed that ChERK expression was detected in all of the examined tissues and stages of embryonic development; its transcript level was significantly induced upon challenge with bacterial pathogens (Vibrio alginolyticus and Staphylococcus haemolyticus) in vivo and PAMPs (lipopolysaccharide and peptidoglycan) in vitro. Moreover, ChERK was mainly located in the cytoplasm of HEIC293T cells. Taken together, these findings may provide novel insights into the functions of molluscan ERKs, especially their roles in response to immune challenge in oyster. (C) 2017 Published by Elsevier Ltd.
部门归属[Qu, Fufa; Xiang, Zhiming; Li, Jun; Xiao, Shu; Mao, Fan; Qin, Yanping; Zhou, Yingli; Ma, Haitao; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Fufa; Xiang, Zhiming; Li, Jun; Xiao, Shu; Mao, Fan; Qin, Yanping; Zhou, Yingli; Ma, Haitao; Yu, Ziniu] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China; [Qu, Fufa] Changsha Univ, Dept Biol & Environm Engn, Changsha 410022, Hunan, Peoples R China
关键词Extracellular Signal-regulated Kinase Expression Patterns Immune Challenge Subcellular Localization Crassostrea Hongkongensis
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16244
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zhimingxiang@scsio.ac.cn; carlzyu@scsio.ac.cn
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Qu, FF,Xiang, ZM,Li, J,et al. A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro[J]. FISH & SHELLFISH IMMUNOLOGY,2017,62:311-319.
APA Qu, FF.,Xiang, ZM.,Li, J.,Xiao, S.,Mao, F.,...&carlzyu@scsio.ac.cn.(2017).A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro.FISH & SHELLFISH IMMUNOLOGY,62,311-319.
MLA Qu, FF,et al."A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro".FISH & SHELLFISH IMMUNOLOGY 62(2017):311-319.
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