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Comparative Characterization of Sequence Structure, Gene Expression, and Enzyme Activity of Alpha-2-macroglobulin between Stichopus monotuberculatus (Stichopodidae) and Holothuria atra (Holothuriidae)
Qian, J; Ren, CH; Xia, JJ; Yu, ZH; Hu, CQ; cqhu@scsio.ac.cn
2017
Source PublicationJOURNAL OF THE WORLD AQUACULTURE SOCIETY
Volume48Issue:2Pages:360-373
AbstractAlpha-2-macroglobulin (A(2)M) is essential for hosts defending against pathogen infections. In this study, two distinct A(2)Ms were identified from sea cucumbers Stichopus monotuberculatus and Holothuria atra, which belong to different genera in the Phylum Echinodermata, and named StmA(2)M and HaA(2)M, respectively. Structural analysis of StmA(2)M and HaA(2)M revealed that all contain the bait region domain, thioester domain, and receptor-binding domain. Phylogenetic analysis indicated that StmA(2)M and HaA(2)M all belong to the echinoderm A(2)Ms. The cleavage site RXXR and the catalytic histidine residue for complement component C3 were found in StmA(2)M and HaA(2)M. Compared with human A(2)M, the three-dimensional structures of StmA(2)M and HaA(2)M were more similar to that of common carp A(2)M. Both StmA(2)M and HaA(2)M showed the strongest response at transcriptional levels to lipopolysaccharide (LPS) at 12h and polyriboinosinic polyribocytidylic acid [Poly (I: C)] at 24h, respectively. Activity of StmA(2)M showed the strongest response to Poly (I: C) at 6h, while the strongest change of HaA(2)M activity was induced by LPS at 24h. The differential mRNA expression and enzyme activity levels suggested that A(2)M in sea cucumbers might be involved in different immune responses to LPS or Poly (I: C) challenge.
Department[Qian, Jing; Ren, Chunhua; Xia, Jianjun; Yu, Zonghe; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Qian, Jing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ren, Chunhua; Hu, Chaoqun] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16258
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcqhu@scsio.ac.cn
Recommended Citation
GB/T 7714
Qian, J,Ren, CH,Xia, JJ,et al. Comparative Characterization of Sequence Structure, Gene Expression, and Enzyme Activity of Alpha-2-macroglobulin between Stichopus monotuberculatus (Stichopodidae) and Holothuria atra (Holothuriidae)[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(2):360-373.
APA Qian, J,Ren, CH,Xia, JJ,Yu, ZH,Hu, CQ,&cqhu@scsio.ac.cn.(2017).Comparative Characterization of Sequence Structure, Gene Expression, and Enzyme Activity of Alpha-2-macroglobulin between Stichopus monotuberculatus (Stichopodidae) and Holothuria atra (Holothuriidae).JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(2),360-373.
MLA Qian, J,et al."Comparative Characterization of Sequence Structure, Gene Expression, and Enzyme Activity of Alpha-2-macroglobulin between Stichopus monotuberculatus (Stichopodidae) and Holothuria atra (Holothuriidae)".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.2(2017):360-373.
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