Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies
Zhou, LL; Li, PF; Liu, JX; Ni, SW; Yu, YP; Yang, M; Wei, SN; Qin, QW; weisn@scsio.ac.cn; qinqiwei64@outlook.com
2017
发表期刊IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
卷号53期号:4页码:320-327
摘要Golden pompano Trachinotus ovatus, a popularly cultured and commercially important marine fish worldwide, has been recognized as a promising candidate for mariculture. However, outbreaks of infectious bacterial or viral diseases and environmental deterioration have led to great economic losses in T. ovatus aquaculture recently. In our research, we established a new mid-kidney cell line, designated as TOK, from golden pompano, T. ovatus. The optimized growth temperature and working concentration of fetal bovine serum (FBS) were 28A degrees C and 10-20%, respectively. Foreign genes could express well in TOK cells. The modal number of TOK cells was 54. The TOK cells were susceptive to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), and the virus could propagate in cells. Propagation was verified by qRT-PCR, and virions were observed under electron microscopy. Cytotoxicity analysis revealed that TOK cells were sensitive to different concentrations of extracellular products (ECPs) from Vibrio alginolyticus and V. anguillarum. Moreover, heavy metals (Cd, Cu, and Hg) also showed dose-dependent cytotoxicity to the TOK cell line. We established a mid-kidney cell line from T. ovatus which could be applied to cytotoxicity assays of heavy metals. The newly established TOK cell line possesses great application potential in genetic manipulation, virus-host interaction studies, and toxicity assays of bacterial extracellular products and heavy metals.
部门归属[Zhou, Lingli; Liu, Jiaxin; Ni, Songwei; Yu, Yepin; Yang, Min; Wei, Shina; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Li, Pengfei] Guangxi Acad Sci, Guangxi Key Lab Marine Nat Prod & Combinatorial B, Nanning 530007, Peoples R China; [Zhou, Lingli; Li, Pengfei; Liu, Jiaxin; Ni, Songwei; Yu, Yepin] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China; [Qin, Qiwei] South China Agr Univ, Coll Marine Sci, 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China
关键词Trachinotus Ovatus Mid-kidney Cell Line Cytotoxicity Nervous Necrosis Virus Heavy Metals
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16259
专题中科院海洋生物资源可持续利用重点实验室
通讯作者weisn@scsio.ac.cn; qinqiwei64@outlook.com
推荐引用方式
GB/T 7714
Zhou, LL,Li, PF,Liu, JX,et al. Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2017,53(4):320-327.
APA Zhou, LL.,Li, PF.,Liu, JX.,Ni, SW.,Yu, YP.,...&qinqiwei64@outlook.com.(2017).Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,53(4),320-327.
MLA Zhou, LL,et al."Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 53.4(2017):320-327.
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