|Identification and functional characterization of first alpha-2-macroglobulin in sea cucumbers|
|Qian, J; Ren, CH; Chen, T; Xia, JJ; Yu, ZH; Gao, Y; Hu, CQ; firstname.lastname@example.org
|Source Publication||AQUACULTURE RESEARCH
|Abstract||The broad-spectrum protease inhibitor alpha-2-macroglobulin (M-2) is widely found in vertebrates and invertebrates. However, no research regarding M-2 has been described in sea cucumbers to date. In this study, the first sea cucumber M-2 named Stm(2)M was identified from Stichopus monotuberculatus, a tropical species with high edible and economic values in Southern China. The Stm(2)M cDNA is 5276bp in length, containing a 5-untranslated region (UTR) of 279bp, a 3-UTR of 344bp and an open reading frame of 4653bp that encodes a protein of 1550 amino acids. The Stm(2)M possesses all the three characteristics of known M-2 proteins, including the bait region domain, thioester domain and receptor-binding domain. Higher levels of mRNA expression were noticed in intestine, respiratory tree, coelomocytes and tentacle of S.monotuberculatus. Transcriptional expression of Stm(2)M showed the strongest response to lipopolysaccharides (LPS, 8.19-fold up-regulation) after 6h post-challenge in coelomocytes. In addition, the M-2 activity was detected in S.monotuberculatus and protein activity of M-2 increased remarkably at 6h after LPS stimulation. As a whole, this study indicated that Stm(2)M is an important immune-related molecule, and the study on functions of Stm(2)M may provide some new and valuable ideas for preventing and controlling diseases of cultured sea cucumbers.|
|Department||[Qian, Jing; Ren, Chunhua; Chen, Ting; Xia, Jianjun; Yu, Zonghe; Gao, Yan; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou, Guangdong, Peoples R China; [Qian, Jing; Gao, Yan] Univ Chinese Acad Sci, Beijing, Peoples R China; [Ren, Chunhua; Chen, Ting; Hu, Chaoqun] South China Sea Bioresource Exploitat & Utilizat, Beijing, Peoples R China
Qian, J,Ren, CH,Chen, T,et al. Identification and functional characterization of first alpha-2-macroglobulin in sea cucumbers[J]. AQUACULTURE RESEARCH,2017,48(5):2278-2290.
Qian, J.,Ren, CH.,Chen, T.,Xia, JJ.,Yu, ZH.,...&email@example.com.(2017).Identification and functional characterization of first alpha-2-macroglobulin in sea cucumbers.AQUACULTURE RESEARCH,48(5),2278-2290.
Qian, J,et al."Identification and functional characterization of first alpha-2-macroglobulin in sea cucumbers".AQUACULTURE RESEARCH 48.5(2017):2278-2290.
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