Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli
Guo, YX; Liu, XX; Li, BY; Yao, JY; Wood, TK; Wang, XX; xxwang@scsio.ac.cn
2017
发表期刊JOURNAL OF BACTERIOLOGY
卷号199期号:9页码:UNSP e00057-
摘要Host-associated bacteria, such as Escherichia coli, often encounter various host-related stresses, such as nutritional deprivation, oxidative stress, and temperature shifts. There is growing interest in searching for small endogenous proteins that mediate stress responses. Here, we characterized the small C-tail-anchored inner membrane protein ElaB in E. coli. ElaB belongs to a class of tail-anchored inner membrane proteins with a C-terminal transmembrane domain but lacking an N-terminal signal sequence for membrane targeting. Proteins from this family have been shown to play vital roles, such as in membrane trafficking and apoptosis, in eukaryotes; however, their role in prokaryotes is largely unexplored. Here, we found that the transcription of elaB is induced in the stationary phase in E. coli and stationary-phase sigma factor RpoS regulates elaB transcription by binding to the promoter of elaB. Moreover, ElaB protects cells against oxidative stress and heat shock stress. However, unlike membrane peptide toxins TisB and GhoT, ElaB does not lead to cell death, and the deletion of elaB greatly increases persister cell formation. Therefore, we demonstrate that disruption of C-tail-anchored inner membrane proteins can reduce stress resistance; it can also lead to deleterious effects, such as increased persistence, in E. coli. IMPORTANCE Escherichia coli synthesizes dozens of poorly understood small membrane proteins containing a predicted transmembrane domain. In this study, we characterized the function of the C-tail-anchored inner membrane protein ElaB in E. coli. ElaB increases resistance to oxidative stress and heat stress, while inactivation of ElaB leads to high persister cell formation. We also demonstrated that the transcription of elaB is under the direct regulation of stationary-phase sigma factor RpoS. Thus, our study reveals that small inner membrane proteins may have important cellular roles during the stress response.
部门归属[Guo, Yunxue; Liu, Xiaoxiao; Li, Baiyuan; Yao, Jianyun; Wang, Xiaoxue] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, Key Lab Trop Marine Bioresources & Ecol,Guangdong, Guangzhou, Guangdong, Peoples R China; [Li, Baiyuan; Yao, Jianyun] Univ Chinese Acad Sci, Beijing, Peoples R China; [Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA; [Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
关键词C-tail-anchored Membrane Protein Oxidative Stress Heat Shock Persistence
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16271
专题中科院海洋生物资源可持续利用重点实验室
通讯作者xxwang@scsio.ac.cn
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GB/T 7714
Guo, YX,Liu, XX,Li, BY,et al. Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli[J]. JOURNAL OF BACTERIOLOGY,2017,199(9):UNSP e00057-.
APA Guo, YX.,Liu, XX.,Li, BY.,Yao, JY.,Wood, TK.,...&xxwang@scsio.ac.cn.(2017).Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli.JOURNAL OF BACTERIOLOGY,199(9),UNSP e00057-.
MLA Guo, YX,et al."Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli".JOURNAL OF BACTERIOLOGY 199.9(2017):UNSP e00057-.
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