SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli
Guo, YX; Liu, XX; Li, BY; Yao, JY; Wood, TK; Wang, XX; xxwang@scsio.ac.cn
2017
Source PublicationJOURNAL OF BACTERIOLOGY
Volume199Issue:9Pages:UNSP e00057-
AbstractHost-associated bacteria, such as Escherichia coli, often encounter various host-related stresses, such as nutritional deprivation, oxidative stress, and temperature shifts. There is growing interest in searching for small endogenous proteins that mediate stress responses. Here, we characterized the small C-tail-anchored inner membrane protein ElaB in E. coli. ElaB belongs to a class of tail-anchored inner membrane proteins with a C-terminal transmembrane domain but lacking an N-terminal signal sequence for membrane targeting. Proteins from this family have been shown to play vital roles, such as in membrane trafficking and apoptosis, in eukaryotes; however, their role in prokaryotes is largely unexplored. Here, we found that the transcription of elaB is induced in the stationary phase in E. coli and stationary-phase sigma factor RpoS regulates elaB transcription by binding to the promoter of elaB. Moreover, ElaB protects cells against oxidative stress and heat shock stress. However, unlike membrane peptide toxins TisB and GhoT, ElaB does not lead to cell death, and the deletion of elaB greatly increases persister cell formation. Therefore, we demonstrate that disruption of C-tail-anchored inner membrane proteins can reduce stress resistance; it can also lead to deleterious effects, such as increased persistence, in E. coli. IMPORTANCE Escherichia coli synthesizes dozens of poorly understood small membrane proteins containing a predicted transmembrane domain. In this study, we characterized the function of the C-tail-anchored inner membrane protein ElaB in E. coli. ElaB increases resistance to oxidative stress and heat stress, while inactivation of ElaB leads to high persister cell formation. We also demonstrated that the transcription of elaB is under the direct regulation of stationary-phase sigma factor RpoS. Thus, our study reveals that small inner membrane proteins may have important cellular roles during the stress response.
Department[Guo, Yunxue; Liu, Xiaoxiao; Li, Baiyuan; Yao, Jianyun; Wang, Xiaoxue] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, Key Lab Trop Marine Bioresources & Ecol,Guangdong, Guangzhou, Guangdong, Peoples R China; [Li, Baiyuan; Yao, Jianyun] Univ Chinese Acad Sci, Beijing, Peoples R China; [Wood, Thomas K.] Penn State Univ, Dept Chem Engn, University Pk, PA 16802 USA; [Wood, Thomas K.] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA
KeywordC-tail-anchored Membrane Protein Oxidative Stress Heat Shock Persistence
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16271
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorxxwang@scsio.ac.cn
Recommended Citation
GB/T 7714
Guo, YX,Liu, XX,Li, BY,et al. Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli[J]. JOURNAL OF BACTERIOLOGY,2017,199(9):UNSP e00057-.
APA Guo, YX.,Liu, XX.,Li, BY.,Yao, JY.,Wood, TK.,...&xxwang@scsio.ac.cn.(2017).Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli.JOURNAL OF BACTERIOLOGY,199(9),UNSP e00057-.
MLA Guo, YX,et al."Tail-Anchored Inner Membrane Protein ElaB Increases Resistance to Stress While Reducing Persistence in Escherichia coli".JOURNAL OF BACTERIOLOGY 199.9(2017):UNSP e00057-.
Files in This Item:
File Name/Size DocType Version Access License
Tail-Anchored Inner (1674KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.