Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells
Shi, S; Guo, KB; Wang, XG; Chen, H; Min, JB; Qi, SH; Zhao, W; Li, WR; liwr@gzucm.edu.cn
2017
发表期刊MOLECULAR MEDICINE REPORTS
卷号15期号:5页码:2611-2619
摘要Oxalicumone A (POA), a novel dihydrothiophene-condensed chromone, was isolated from the marine-derived fungus Penicillium oxalicum. Previous reports demonstrated that POA exhibits strong activity against human carcinoma cells, thus it has been suggested as a bioactive anticancer agent. To research the toxic effect of POA on cultured normal epithelial human kidney-2 (HK-2) cells and evaluate its clinical safety, cell survival was evaluated by the Cell Counting Kit-8 assay and apoptosis was evaluated by Hoechst 33258 staining, flow cytometry, caspase-3 activity assay and western blotting. 2',7'-Dichlorofluorescin diacetate and JC-1 dye staining was used to evaluate reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP), respectively. The results indicated that POA inhibited HK-2 cell growth and promoted apoptosis, by increasing levels of Fas cell surface cell receptor and the B-cell lymphoma 2 associated protein X apoptosis regulator (Bax)/B-cell lymphoma 2 apoptosis regulator (Bcl-2) ratio. POA treatment also induced release of ROS and loss of MMP in HK-2 cells. Compared with untreated control, a significant decrease was also demonstrated in superoxide dismutase activity and glutathione content with POA treatment, accompanied by enhanced release of N-acetyl-beta-D-glucosaminidase, increased leakage of lactate dehydrogenase, increased malondialdehyde formation and increased release of nitric oxide. In conclusion, the present in vitro study revealed that POA exhibits antiproliferation activity on HK-2 cells, through stimulation of apoptosis and oxidative stress injury, which may be relevant to its clinical application. The present study may, therefore, offer valuable new information regarding the use of POA as a candidate novel antitumor drug for clinical use.
部门归属[Shi, Si; Guo, Kunbin; Wang, Xiangyu; Chen, Hao; Min, Jianbin; Zhao, Wei; Li, Weirong] Guangzhou Univ Chinese Med, Inst Clin Pharmacol, 12 Jichang Rd, Guangzhou 510405, Guangdong, Peoples R China; [Qi, Shuhua] Chinese Acad Sci, Guangdong Key Lab Marine Mat Med, Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
关键词Penicillium Oxalicum Oxalicumone a Human Kidney-2 Cells Cytotoxicity Apoptosis Mitochondria
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16277
专题中科院海洋生物资源可持续利用重点实验室
通讯作者liwr@gzucm.edu.cn
推荐引用方式
GB/T 7714
Shi, S,Guo, KB,Wang, XG,et al. Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells[J]. MOLECULAR MEDICINE REPORTS,2017,15(5):2611-2619.
APA Shi, S.,Guo, KB.,Wang, XG.,Chen, H.,Min, JB.,...&liwr@gzucm.edu.cn.(2017).Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells.MOLECULAR MEDICINE REPORTS,15(5),2611-2619.
MLA Shi, S,et al."Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells".MOLECULAR MEDICINE REPORTS 15.5(2017):2611-2619.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
MOLECULAR MEDICINE R(1472KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。