Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility
Li, P; Zhou, L; Wei, S; Yang, M; Ni, S; Yu, Y; Cai, J; Qin, Q; qinqw@scsio.ac.cn
2017
发表期刊JOURNAL OF FISH BIOLOGY
卷号90期号:5页码:1944-1959
摘要A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28 degrees C and the optimum foetal bovine serum concentration in L-15 medium was 10%. The TOHK cells have a diploid chromosome number of 2N = 54. The transfection efficiency of TOHK cells was 75% at the 15th passage and 72% at the 40th passage. The transfection efficiency in TOHK cells was high, so these cells are suitable for foreign gene expression. The cytotoxic effects of heavy metals and extracellular products from Vibrio anguillarum and Vibrio alginolyticus were demonstrated in TOHK cells, so this TOHK cell line could also be applied in environmental monitoring of heavy metals and pathogenic bacteria. TOHK cell line showed high virus susceptibility, such as grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). Then, TOHK cell line could be used for the study of viral pathogenesis and the development of antiviral strategies.
部门归属[Li, P.] Guangxi Acad Sci, Guangxi Key Lab Marine Nat Prod & Combinatorial B, Nanning 530007, Peoples R China; [Li, P.; Zhou, L.; Wei, S.; Ni, S.; Yu, Y.; Qin, Q.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Zhou, L.; Ni, S.; Yu, Y.] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang, M.; Qin, Q.] South China Agr Univ, Coll Marine Sci, Guangzhou, Guangdong, Peoples R China; [Cai, J.] Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China
关键词Cytotoxicity Head-kidney Cell Line Trachinotus Ovatus Transfection Efficiency Viral Susceptibility
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16278
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Li, P,Zhou, L,Wei, S,et al. Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility[J]. JOURNAL OF FISH BIOLOGY,2017,90(5):1944-1959.
APA Li, P.,Zhou, L.,Wei, S.,Yang, M.,Ni, S.,...&qinqw@scsio.ac.cn.(2017).Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility.JOURNAL OF FISH BIOLOGY,90(5),1944-1959.
MLA Li, P,et al."Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility".JOURNAL OF FISH BIOLOGY 90.5(2017):1944-1959.
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