Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni
Gao, BL; Vorwerk, H; Huber, C; Lara-Tejero, M; Mohr, J; Goodman, AL; Eisenreich, W; Galan, JE; Hofreuter, D; jorge.galan@yale.edu; dirk.hofreuter@gmail.com
2017
发表期刊PLOS BIOLOGY
卷号15期号:5页码:e2001390-
摘要Campylobacter jejuni is one of the leading infectious causes of food-borne illness around the world. Its ability to persistently colonize the intestinal tract of a broad range of hosts, including food-producing animals, is central to its epidemiology since most infections are due to the consumption of contaminated food products. Using a highly saturated transposon insertion library combined with next-generation sequencing and a mouse model of infection, we have carried out a comprehensive genome-wide analysis of the fitness determinants for growth in vitro and in vivo of a highly pathogenic strain of C. jejuni. A comparison of the C. jejuni requirements to colonize the mouse intestine with those necessary to grow in different culture media in vitro, combined with isotopologue profiling and metabolic flow analysis, allowed us to identify its metabolic requirements to establish infection, including the ability to acquire certain nutrients, metabolize specific substrates, or maintain intracellular ion homeostasis. This comprehensive analysis has identified metabolic pathways that could provide the basis for the development of novel strategies to prevent C. jejuni colonization of food-producing animals or to treat human infections.
部门归属[Gao, Beile; Lara-Tejero, Maria; Goodman, Andrew L.; Galan, Jorge E.] Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT USA; [Vorwerk, Hanne; Mohr, Juliane; Hofreuter, Dirk] Hannover Med Sch, Inst Med Microbiol & Hosp Epidemiol, Hannover, Germany; [Huber, Claudia; Eisenreich, Wolfgang] Tech Univ Munich, Lehrstuhl Biochem, Garching, Germany; [Goodman, Andrew L.] Yale Univ, Sch Med, Microbial Sci Inst, New Haven, CT USA; [Gao, Beile] Chinese Acad Sci, CAS Key Lab Trop Marine Bio Resources & Ecol, Guangdong Key Lab Marine Mat Med, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Mohr, Juliane] Friedrich Schiller Univ Jena, Klin Hamatol & Internist Onkol, Jena, Germany
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16281
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jorge.galan@yale.edu; dirk.hofreuter@gmail.com
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Gao, BL,Vorwerk, H,Huber, C,et al. Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni[J]. PLOS BIOLOGY,2017,15(5):e2001390-.
APA Gao, BL.,Vorwerk, H.,Huber, C.,Lara-Tejero, M.,Mohr, J.,...&dirk.hofreuter@gmail.com.(2017).Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni.PLOS BIOLOGY,15(5),e2001390-.
MLA Gao, BL,et al."Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni".PLOS BIOLOGY 15.5(2017):e2001390-.
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