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MqsR/MqsA Toxin/Antitoxin System Regulates Persistence and Biofilm Formation in Pseudomonas putida KT2440
Sun, CL; Guo, YX; Tang, KH; Wen, ZL; Li, BY; Zeng, ZS; Wang, XX; xxwang@scsio.ac.cn
2017
Source PublicationFRONTIERS IN MICROBIOLOGY
Volume8Pages:840-
AbstractBacterial toxin/antitoxin (TA) systems have received increasing attention due to their prevalence, diverse structures, and important physiological functions. In this study, we identified and characterized a type II TA system in a soil bacterium Pseudomonas putida KT2440. This TA system belongs to the MqsR/MqsA family. We found that PP_4205 (MqsR) greatly inhibits cell growth in P. putida KT2440 and Escherichia coli, the antitoxin PP_4204 (MqsA) neutralizes the toxicity of the toxin MqsR, and the two genes encoding them are co-transcribed. MqsR and MqsA interact with each other directly in vivo and MqsA is a negative regulator of the TA operon through binding to the promoter. Consistent with the MqsR/MqsA pair in E. coli, the binding of the toxin MqsR to MqsA inhibits the DNA binding ability of MqsA in P. putida KT2440. Disruption of the mqsA gene which induces mqsR expression increases persister cell formation 53-fold, while overexpressing mqsA which represses mqsR expression reduces persister cell formation 220-fold, suggesting an important role of MqsR in persistence in P. putida KT2440. Furthermore, both MqsR and MqsA promote biofilm formation. As a DNA binding protein, MqsA can also negatively regulate an ECF sigma factor AlgU and a universal stress protein PP_3288. Thus, we revealed an important regulatory role of MqsR/MqsA in persistence and biofilm formation in P. putida KT2440.
Department[Sun, Chenglong; Guo, Yunxue; Tang, Kaihao; Wen, Zhongling; Li, Baiyuan; Zeng, Zhenshun; Wang, Xiaoxue] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol,Guangdong Key Lab Marin, Guangzhou, Peoples R China; [Sun, Chenglong; Wen, Zhongling] Univ Chinese Acad Sci, Beijing, Peoples R China
KeywordToxin/antitoxin System Mqsr/mqsa Biofilm Persistence Pseudomonas Putida
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16285
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorxxwang@scsio.ac.cn
Recommended Citation
GB/T 7714
Sun, CL,Guo, YX,Tang, KH,et al. MqsR/MqsA Toxin/Antitoxin System Regulates Persistence and Biofilm Formation in Pseudomonas putida KT2440[J]. FRONTIERS IN MICROBIOLOGY,2017,8:840-.
APA Sun, CL.,Guo, YX.,Tang, KH.,Wen, ZL.,Li, BY.,...&xxwang@scsio.ac.cn.(2017).MqsR/MqsA Toxin/Antitoxin System Regulates Persistence and Biofilm Formation in Pseudomonas putida KT2440.FRONTIERS IN MICROBIOLOGY,8,840-.
MLA Sun, CL,et al."MqsR/MqsA Toxin/Antitoxin System Regulates Persistence and Biofilm Formation in Pseudomonas putida KT2440".FRONTIERS IN MICROBIOLOGY 8(2017):840-.
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