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A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi
Tian, YS; Chen, T; Luo, P; Huang, W; Huo, D; Yun, L; Hu, CQ; Cheng, CH; luopeng@scsio.ac.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume78Pages:364-371
AbstractFibrinogen-related proteins (FREPs) play a crucial role in invertebrate immune response. In this study, we acquired a novel fibrinogen-related protein gene in Litopenaeus vannamei coding for one kind of fibrinogen-related protein, designated as LvFREP2. The complete cDNA sequence of LvFREP2 was 1903 bp long, containing an open reading frame of 1479 bp coding for LvFREP2. The LvFREP2 protein contained a putative signal peptide and a fibrinogen-related protein domain. qRT-PCRs indicated that LvFREP2 mRNA ubiquitously distributed in all examined tissues, and it was up-regulated in gills after V. harveyi and LPS challenges. The recombinant LvFREP2 agglutinated Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (Vibrio alginolyticus, V. cholerae, V. vulnificus, V. parahaemolyticus, V. harveyi, Pseudomonas aeruginosa, P. fluorescens) in a calcium-dependent manner. LvFREP2 also facilitated the clearance of Vibrio harveyi in vivo. Therefore, our results suggested that lvFREP2 may have important roles in the anti-bacterial immunity of L. vannamei.
Department[Tian, Yushun; Chen, Ting; Luo, Peng; Huang, Wen; Huo, Da; Yun, Long; Hu, Chaoqun; Cheng, Chuhang] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 501301, Guangdong, Peoples R China; [Tian, Yushun; Huo, Da; Yun, Long] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Ting; Luo, Peng; Huang, Wen; Hu, Chaoqun; Cheng, Chuhang] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510301, Guangdong, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordLitopenaeus vannamei ; Fibrinogen-related proteins ; Vibrio harveyi ; Innate immunity
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16924
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorluopeng@scsio.ac.cn
Recommended Citation
GB/T 7714
Tian, YS,Chen, T,Luo, P,et al. A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi[J]. FISH & SHELLFISH IMMUNOLOGY,2018,78:364-371.
APA Tian, YS.,Chen, T.,Luo, P.,Huang, W.,Huo, D.,...&luopeng@scsio.ac.cn.(2018).A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi.FISH & SHELLFISH IMMUNOLOGY,78,364-371.
MLA Tian, YS,et al."A fibrinogen-related protein, LvFREP2, from Litopenaeus vannamei facilitates the clearance of Vibrio harveyi".FISH & SHELLFISH IMMUNOLOGY 78(2018):364-371.
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