Vibrio alginolyticus 16S-23S intergenic spacer region analysis, and PCR assay for identification of coral pathogenic strain XSBZ03
Li, HY; Zhang, X; Long, H; Hu, CQ; Zhou, YC; Wang, SF; Ke, SW; Xie, ZY; xiezyscuta@163.com
2018
发表期刊DISEASES OF AQUATIC ORGANISMS
卷号129期号:1页码:71-83
摘要Porites andrewsi white syndrome (PAWS), caused by Vibrio alginolyticus strains XSBZ03 and XSBZ14, poses a serious threat to corals in the South China Sea. To obtain a specific target against which to develop a rapid PCR detection method for the coral pathogenic strain XSBZ03, the 16S-23S rRNA gene intergenic spacer (IGS) region of 4 strains of V. alginolyticus, including the XSBZ03 and XSBZ14 strains, was amplified, sequenced and analyzed. Six types of IGS were found: IGS(0), IGS(G), IGS(IA), IGS(AG), IGS(GLV), and IGS(GLAV). IGS(0), IGS(G), IGS(IA), IGS(AG) and IGS(GLV) appeared to be the most prevalent forms in the 4 strains and the percentage identity range within each type was 91.4-100%, 89.3-98.5%, 83.0-99.8%, 91.5-95.6%, and 88.7-99.3%, respectively. IGS(GLAV) was found only in the HN08155 strain, a causative agent of fish disease. IGS(GLAV), IGS(GLV) and IGS(AG) are reported here for the first time in V. alginolyticus. An IGS sequence specific to the XSBZ03 strain was identified following alignment of the homologous IGSs, and used to design strain-specific primers for its rapid identification by PCR. The results from PCR analysis suggest that the method is a rapid, practical, and reliable tool for the identification of the XSBZ03 strain in samples of isolated bacteria, as well as seawater and coral samples spiked with the bacterial strain. This is the first report of a rapid diagnostic assay for a causative agent of PAWS, based on PCR detection of a coral pathogen at the strain level. After applying this assay in coral transplantation, the survival rates of transplanted corals were significantly increased. This diagnostic assay should aid with both the elucidation of the cause of the disease, and transplantation of PAWS-free P. andrewsi in the South China Sea.
部门归属[Li, Hongyue; Zhang, Xiang; Long, Hao; Zhou, Yongcan; Wang, Shifeng; Ke, Shaowen; Xie, Zhenyu] Hainan Univ, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China; [Li, Hongyue; Zhang, Xiang; Zhou, Yongcan; Wang, Shifeng; Ke, Shaowen; Xie, Zhenyu] Hainan Univ, Hainan Prov Key Lab Trop Hydrobiol & Biotechnol, Haikou 570228, Hainan, Peoples R China; [Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Marine Bioresources Sustainable Utili, Guangzhou 510301, Guangdong, Peoples R China
学科领域Fisheries ; Veterinary Sciences
关键词[WOS]Porites andrewsi white syndrome ; Vibrio alginolyticus ; Intergenic spacer ; IGS ; Strain-specific primers ; Diagnostic protocol
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16927
专题中科院海洋生物资源可持续利用重点实验室
通讯作者xiezyscuta@163.com
推荐引用方式
GB/T 7714
Li, HY,Zhang, X,Long, H,et al. Vibrio alginolyticus 16S-23S intergenic spacer region analysis, and PCR assay for identification of coral pathogenic strain XSBZ03[J]. DISEASES OF AQUATIC ORGANISMS,2018,129(1):71-83.
APA Li, HY.,Zhang, X.,Long, H.,Hu, CQ.,Zhou, YC.,...&xiezyscuta@163.com.(2018).Vibrio alginolyticus 16S-23S intergenic spacer region analysis, and PCR assay for identification of coral pathogenic strain XSBZ03.DISEASES OF AQUATIC ORGANISMS,129(1),71-83.
MLA Li, HY,et al."Vibrio alginolyticus 16S-23S intergenic spacer region analysis, and PCR assay for identification of coral pathogenic strain XSBZ03".DISEASES OF AQUATIC ORGANISMS 129.1(2018):71-83.
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