Biocompatible Surface-Coated Probe for in Vivo, in Situ, and Microscale Lipidomics of Small Biological Organisms and Cells Using Mass Spectrometry
Deng, JW; Li, WY; Yang, QX; Liu, YH; Fang, L; Guo, YH; Guo, PR; Lin, L; Yang, YY; Luan, TG; yy_yang@vip.126.com; cesltg@mail.sysu.edu.cn
2018
发表期刊ANALYTICAL CHEMISTRY
卷号90期号:11页码:6936-6944
摘要Lipidomics is a significant way to understand the structural and functional roles that lipids play in biological systems. Although many mass spectrometry (MS)-based lipidomics strategies have recently achieve remarkable results, in vivo, in situ, and microscale lipidomics for small biological organisms and cells have not yet been obtained. In this article, we report a novel lipidomics methodology for in vivo, in situ, and microscale investigation of small biological organisms and cells using biocompatible surface-coated probe nanoelectrospray ionization mass spectrometry (BSCP-nanoESI-MS). A novel biocompatible surface-coated solid-phase microextration (SPME) probe is prepared, which possesses a probe-end diameter of less than 5 pim and shows excellent enrichment capacity toward lipid species. In vivo extraction of living biological organisms (e.g., zebrafishes), in situ sampling a precise position of small organisms (e.g., Daphnia magna), and even microscale analysis of single eukaryotic cells (e.g., HepG2) are easily achieved by the SPME probe. After extraction, the loaded SPME probe is directly applied for nanoESI-MS analysis, and a high-resolution mass spectrometer is employed for recording spectra and identifying lipid species. Compared with the conventional direct infusion shotgun MS lipidomics, our proposed methodology shows a similar result of lipid profiles but with simpler sample pretreatment, less sample consumption, and shorter analytical times. Lipidomics of zebrafish, Daphnia magna, and HepG2 cell populations were investigated by our proposed BSCP-nanoESl-MS methodology, and abundant lipid compositions were detected and identified and biomarkers were obtained via multivariate statistical analysis.
部门归属[Deng, Jiewei; Li, Wenying; Fang, Ling; Lin, Li; Luan, Tiangang] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, South China Sea Bioresource Exploitat & Utilizat, 135 Xingangxi Rd, Guangzhou 510275, Guangdong, Peoples R China; [Yang, Qiuxia; Liu, Yaohui; Guo, Pengran; Yang, Yunyun] China Natl Analyt Ctr Guangzhou, Guangdong Inst Anal, Guangdong Engn & Technol Res Ctr Ambient Mass Spe, Guangdong Prov Key Lab Emergency Test Dangerous C, 100 Xianlie Middle Rd, Guangzhou 510070, Guangdong, Peoples R China; [Fang, Ling; Guo, Yunhua] Sun Yat Sen Univ, Instrumental Anal & Res Ctr, 135 Xingangxi Rd, Guangzhou 510275, Guangdong, Peoples R China
学科领域Chemistry
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16930
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yy_yang@vip.126.com; cesltg@mail.sysu.edu.cn
推荐引用方式
GB/T 7714
Deng, JW,Li, WY,Yang, QX,et al. Biocompatible Surface-Coated Probe for in Vivo, in Situ, and Microscale Lipidomics of Small Biological Organisms and Cells Using Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2018,90(11):6936-6944.
APA Deng, JW.,Li, WY.,Yang, QX.,Liu, YH.,Fang, L.,...&cesltg@mail.sysu.edu.cn.(2018).Biocompatible Surface-Coated Probe for in Vivo, in Situ, and Microscale Lipidomics of Small Biological Organisms and Cells Using Mass Spectrometry.ANALYTICAL CHEMISTRY,90(11),6936-6944.
MLA Deng, JW,et al."Biocompatible Surface-Coated Probe for in Vivo, in Situ, and Microscale Lipidomics of Small Biological Organisms and Cells Using Mass Spectrometry".ANALYTICAL CHEMISTRY 90.11(2018):6936-6944.
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