Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate
Wang, YL; Xu, YK; Zhang, Y; Sun, AJ; Hu, YF; yunfeng.hu@scsio.ac.cn
2018
发表期刊CHIRALITY
卷号30期号:6页码:769-776
摘要The two enantiomers of ethyl 3-hydroxybutyrate are important intermediates for the synthesis of a great variety of valuable chiral drugs. The preparation of chiral drug intermediates through kinetic resolution reactions catalyzed by esterases/lipases has been demonstrated to be an efficient and environmentally friendly method. We previously functionally characterized microbial esterase PHE21 and used PHE21 as a biocatalyst to generate optically pure ethyl (S)-3-hydroxybutyrate. Herein, we also functionally characterized one novel salt-tolerant microbial esterase WDEst17 from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. Esterase WDEst17 was further developed as an efficient biocatalyst to generate (R)-3-hydroxybutyrate, an important chiral drug intermediate, with the enantiomeric excess being 99% and the conversion rate being 65.05%, respectively, after process optimization. Notably, the enantio-selectivity of esterase WDEst17 was opposite than that of esterase PHE21. The identification of esterases WDEst17 and PHE21 through genome mining of microorganisms provides useful biocatalysts for the preparation of valuable chiral drug intermediates.
部门归属[Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou, Guangdong, Peoples R China; [Xu, Yongkai] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Jinan, Shandong, Peoples R China; [Hu, Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China
学科领域Pharmacology & Pharmacy ; Chemistry
关键词[WOS]ethyl (R)-3-hydroxybutyrate ; kinetic resolution ; novel esterase ; opposite enantio-selectivity ; process optimization
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16935
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yunfeng.hu@scsio.ac.cn
推荐引用方式
GB/T 7714
Wang, YL,Xu, YK,Zhang, Y,et al. Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate[J]. CHIRALITY,2018,30(6):769-776.
APA Wang, YL,Xu, YK,Zhang, Y,Sun, AJ,Hu, YF,&yunfeng.hu@scsio.ac.cn.(2018).Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate.CHIRALITY,30(6),769-776.
MLA Wang, YL,et al."Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate".CHIRALITY 30.6(2018):769-776.
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