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Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate
Wang, YL; Xu, YK; Zhang, Y; Sun, AJ; Hu, YF; yunfeng.hu@scsio.ac.cn
2018
Source PublicationCHIRALITY
Volume30Issue:6Pages:769-776
AbstractThe two enantiomers of ethyl 3-hydroxybutyrate are important intermediates for the synthesis of a great variety of valuable chiral drugs. The preparation of chiral drug intermediates through kinetic resolution reactions catalyzed by esterases/lipases has been demonstrated to be an efficient and environmentally friendly method. We previously functionally characterized microbial esterase PHE21 and used PHE21 as a biocatalyst to generate optically pure ethyl (S)-3-hydroxybutyrate. Herein, we also functionally characterized one novel salt-tolerant microbial esterase WDEst17 from the genome of Dactylosporangium aurantiacum subsp. Hamdenensis NRRL 18085. Esterase WDEst17 was further developed as an efficient biocatalyst to generate (R)-3-hydroxybutyrate, an important chiral drug intermediate, with the enantiomeric excess being 99% and the conversion rate being 65.05%, respectively, after process optimization. Notably, the enantio-selectivity of esterase WDEst17 was opposite than that of esterase PHE21. The identification of esterases WDEst17 and PHE21 through genome mining of microorganisms provides useful biocatalysts for the preparation of valuable chiral drug intermediates.
Department[Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Wang, Yilong; Zhang, Yun; Sun, Aijun; Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou, Guangdong, Peoples R China; [Xu, Yongkai] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Jinan, Shandong, Peoples R China; [Hu, Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China
Subject AreaPharmacology & Pharmacy ; Chemistry
WOS Keywordethyl (R)-3-hydroxybutyrate ; kinetic resolution ; novel esterase ; opposite enantio-selectivity ; process optimization
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16935
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authoryunfeng.hu@scsio.ac.cn
Recommended Citation
GB/T 7714
Wang, YL,Xu, YK,Zhang, Y,et al. Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate[J]. CHIRALITY,2018,30(6):769-776.
APA Wang, YL,Xu, YK,Zhang, Y,Sun, AJ,Hu, YF,&yunfeng.hu@scsio.ac.cn.(2018).Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate.CHIRALITY,30(6),769-776.
MLA Wang, YL,et al."Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate".CHIRALITY 30.6(2018):769-776.
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