The Molecular Mechanism Underlying Pro-apoptotic Role of Hemocytes Specific Transcriptional Factor Lhx9 in Crassostrea hongkongensis
Zhou, YL; Mao, F; He, ZY; Li, J; Zhang, YH; Xiang, ZM; Xiao, S; Ma, HT; Zhang, Y; Yu, ZN; yzhang@scsio.ac.cn; carlzyu@scsio.ac.cn
2018
发表期刊FRONTIERS IN PHYSIOLOGY
卷号9页码:-612
摘要Hemocytes are the central organ of immune defense against pathogens by means of inflammation, phagocytosis, and encapsulation in mollusks. The well-functioning of the host immune system relies on the hemocytes' task exertion and frequent renewal, but the underlying renewal mechanism remains elusive at the gene level. Here, we identified one transcription factor, LIM homeobox 9, in Crassostrea hongkongensis (ChLhx9) that could be involved in hemocyte apoptosis or renewal. ChLhx9 contains a homeodomain and two LIM domains. The expression profile of ChLhx9 showed that it was specific and had high expression in hemocytes, and it significantly increased under the bacterial challenge. RNA interference of ChLhx9 dramatically decreased the apoptosis rate of hemocytes when compared with a control group, which strongly implies its pro-apoptotic role in hemocytes. Furthermore, the genomic responses to the knockdown of ChLhx9 were examined through RNA-seq, which showed that multiple pathways associated with cell apoptosis, including the apoptosis pathway, hippo signal pathway and p53 signaling pathway, were significantly down-regulated. Meanwhile, seven of the key apoptotic genes were confirmed to be upregulated by ChLhx9, among which ChASPP1 (apoptosis stimulating protein of p53) was confirmed to induce hemocyte apoptosis strongly, which demonstrates that ChASPP1 was a downstream target mediated by ChLhx9 that caused apoptosis. In conclusion, tissue-specific transcription factor ChLhx9 induces hemocyte apoptosis through activating apoptotic genes or pathways, which could contribute to hemocyte renewal and immune defense in oysters.
部门归属[Zhou, Yingli; Mao, Fan; He, Zhiying; Li, Jun; Zhang, Yuehuan; Xiang, Zhiming; Xiao, Shu; Ma, Haitao; Zhang, Yang; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China; [Zhou, Yingli; Mao, Fan; He, Zhiying] Univ Chinese Acad Sci, Beijing, Peoples R China
学科领域Physiology
关键词[WOS]Crassostrea hongkongensis ; hemocytes ; apoptosis ; ChLhx9 ; ChASPP1 ; RNAi ; RNA-seq
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16942
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yzhang@scsio.ac.cn; carlzyu@scsio.ac.cn
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GB/T 7714
Zhou, YL,Mao, F,He, ZY,et al. The Molecular Mechanism Underlying Pro-apoptotic Role of Hemocytes Specific Transcriptional Factor Lhx9 in Crassostrea hongkongensis[J]. FRONTIERS IN PHYSIOLOGY,2018,9:-612.
APA Zhou, YL.,Mao, F.,He, ZY.,Li, J.,Zhang, YH.,...&carlzyu@scsio.ac.cn.(2018).The Molecular Mechanism Underlying Pro-apoptotic Role of Hemocytes Specific Transcriptional Factor Lhx9 in Crassostrea hongkongensis.FRONTIERS IN PHYSIOLOGY,9,-612.
MLA Zhou, YL,et al."The Molecular Mechanism Underlying Pro-apoptotic Role of Hemocytes Specific Transcriptional Factor Lhx9 in Crassostrea hongkongensis".FRONTIERS IN PHYSIOLOGY 9(2018):-612.
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