HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction
Dong, DJ; Zhang, Y; Smykal, V; Ling, L; Raikhel, AS; alexander.raikhel@ucr.edu
2018
发表期刊INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷号96页码:19-26
摘要The Aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. Carbohydrate metabolism (CM) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. The steroid hormone 20-hydroxyecdysone (20E) and the ecdysone receptor (EcR) are important regulators of CM, coordinating it with female reproductive events. We report here that the NR4A nuclear receptor AHR38 plays a critical role in mediating these actions of 20E and EcR. AHR38 RNA interference (RNAi) depletion in female mosquitoes blocked the transcriptional activation of CM genes encoding phosphoglucomutase (PGM) and trehalose-6-phophate synthase (TPS); it caused an increase of glycogen accumulation and a decrease of the circulating sugar trehalose. This treatment also resulted in a dramatic reduction in fecundity. Considering that these phenotypes resulting from AHR38 RNAi depletion are similar to those of EcR RNAi, we investigated a possible connection between these transcription factors in CM regulation. EcR RNAi inhibits the AHR38 gene expression. Moreover, the 20E-induced EcR complex directly activates AHR38 by binding to the ecdysone response element (EcRE) in the upstream regulatory region of this gene. The present work has implicated AHR38 in the 20E-mediated control of CM and provided new insight into mechanisms of 20E regulation of metabolism during female mosquito reproduction.
部门归属[Dong, Dujuan; Zhang, Yang; Smykal, Vlastimil; Ling, Lin; Raikhel, Alexander S.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA; [Dong, Dujuan] Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Biol, Jinan 250100, Shandong, Peoples R China; [Zhang, Yang] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Raikhel, Alexander S.] Univ Calif Riverside, Inst Integrat Genom Biol, Riverside, CA 92521 USA
学科领域Biochemistry & Molecular Biology ; Entomology
关键词[WOS]Hormone ; Nuclear receptor ; Metabolism ; Mosquito ; Disease vector
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16952
专题中科院海洋生物资源可持续利用重点实验室
通讯作者alexander.raikhel@ucr.edu
推荐引用方式
GB/T 7714
Dong, DJ,Zhang, Y,Smykal, V,et al. HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction[J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,2018,96:19-26.
APA Dong, DJ,Zhang, Y,Smykal, V,Ling, L,Raikhel, AS,&alexander.raikhel@ucr.edu.(2018).HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY,96,19-26.
MLA Dong, DJ,et al."HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction".INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 96(2018):19-26.
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