Dual roles of cystatin A in the immune defense of the pacific oyster, Crassostrea gigas
Mao, F; Lin, Y; He, ZY; Li, J; Xiang, ZM; Zhang, Y; Yu, ZN; yzhang@scsio.ac.cn; carlzyu@scsio.ac.cn
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号75页码:190-197
摘要Cystatins are a large family of the proteins that function as reversible and tight-binding inhibitors of cysteine proteases, which consequently regulate multiple physiological activities including apoptosis and innate immunity. In the present study, we cloned a gene from Crassostrea gigas encoding cystatin, which is related to cystatin A superfamily. CgCytA was comprised of a cystatin-like domain with two conserved glycine residues (GG) near the N-terminal and a highly conserved glutamine-valine-glycine (Q-X-V-X-G) motif in the form of QVVAG loop. Transcription analysis of CgCytA indicated its constitutive expression in all tissues including mantle, gill, digestive tract, hemocytes, heart, adductor muscle, and gonads. Immune challenge with Vibrio alginolyticus, resulted in significant down-regulation of CgCytA expression at the initial stages of infection (till 12 h post infection) and the expression of cystatin increased 48 h post infection. Protease assay demonstrated the concentration of cystatin needed to inhibit half of the maximum biological response of cysteine protease is 14.4 mu g/L (IC50). Furthermore, RNAi of CgCytA resulted in increase of apoptotic cell population in hemocytes of C. gigas, suggesting protection role of CgCytA from hemocytes apoptosis. Unexpectedly, knockdown of CgCytA leaded to enhancement of bacterial clearance in vivo, implying that CgCytA may negatively regulate immune defense by suppressing endogenous cysteine protease. Therefore, CgCytA plays dual roles in protection of host hemocytes from apoptosis and control of bacterial clearance, which may server as one of key endogenous balancer between apoptosis and innate immunity in oyster.
部门归属[Mao, Fan; Lin, Yue; He, Zhiying; Li, Jun; Xiang, Zhiming; Zhang, Yang; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Mao, Fan; Lin, Yue; He, Zhiying] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]Crassostrea gigas ; Cysteine proteases ; Cystatin A ; Bacteria defense
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16975
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yzhang@scsio.ac.cn; carlzyu@scsio.ac.cn
推荐引用方式
GB/T 7714
Mao, F,Lin, Y,He, ZY,et al. Dual roles of cystatin A in the immune defense of the pacific oyster, Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2018,75:190-197.
APA Mao, F.,Lin, Y.,He, ZY.,Li, J.,Xiang, ZM.,...&carlzyu@scsio.ac.cn.(2018).Dual roles of cystatin A in the immune defense of the pacific oyster, Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,75,190-197.
MLA Mao, F,et al."Dual roles of cystatin A in the immune defense of the pacific oyster, Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 75(2018):190-197.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Dual roles of cystat(1050KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。