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Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection
Zang, SQ; Zhang, X; Zhang, JC; Li, C; Wei, JG; Qin, QW; weijg@scau.edu.cn; qinqw@scau.edu.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume75Pages:365-373
AbstractThe eukaryotic initiation factor 2 alpha subunit (eIF2 alpha) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2 alpha homolog (EcelF2 alpha) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EcelF2 alpha encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with elF2 alpha s from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EcelF2 alpha was distributed in all examined tissues. Both of the expression levels of EceIF2 alpha in the spleen and head kidney of E. coioides were up-regulated when challenged with polyinosine-polycytidylic acid (poly[I:C]). EceIF2 alpha was abundantly distributed in both the cytoplasm and nucleus in grouper spleen (GS) cells. Over-expression of EceIF2 alpha improved the expression of red-spotted grouper nervous necrosis virus (RGNNV) genes in GS cells. In addition, EceIF2 alpha depressed the activation of NK-kappa B and IFN-beta. Furthermore, dephosphorylation inhibitor treatment led to a significant decrease of RGNNV gene transcription. Taken together, these results suggest that EcelF2 alpha might be involved in the fish immune response to virus challenge.
Department[Zang, Shaoqing; Zhang, Jingcheng; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Zang, Shaoqing; Zhang, Jingcheng; Qin, Qiwei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Xin; Li, Chen; Wei, Jingguang; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Qin, Qiwei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China; [Zang, Shaoqing; Zhang, Jingcheng; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordelF2 alpha ; Phosphorylation ; Epinephelus coioides ; RGNNV
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16976
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorweijg@scau.edu.cn; qinqw@scau.edu.cn
Recommended Citation
GB/T 7714
Zang, SQ,Zhang, X,Zhang, JC,et al. Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection[J]. FISH & SHELLFISH IMMUNOLOGY,2018,75:365-373.
APA Zang, SQ.,Zhang, X.,Zhang, JC.,Li, C.,Wei, JG.,...&qinqw@scau.edu.cn.(2018).Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection.FISH & SHELLFISH IMMUNOLOGY,75,365-373.
MLA Zang, SQ,et al."Involvement of eIF2 alpha of Epinephelus coioides in the fish immune response to virus infection".FISH & SHELLFISH IMMUNOLOGY 75(2018):365-373.
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