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Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure
Zhu, LM; Gao, N; Wang, RF; Zhang, L; zhangli@scsio.ac.cn
2018
Source PublicationECOTOXICOLOGY
Volume27Issue:3Pages:267-277
AbstractAmmonia is both a highly toxic environmental pollutant and the major nitrogenous waste produced by ammoniotelic teleosts. Although the acute toxic effects of ammonia have been widely studied in fish, the biochemical mechanisms of its toxicity have not been understood comprehensively. In this study, we performed comparative proteomic and metabolomic analysis between ammonia-challenged (1.2 and 2.6 mmol L-1 NH4Cl for 96 h) and control groups of marine medaka (Oryzias melastigma) to identify changes of the metabolite and protein profiles in response to ammonia stress. The metabolic responses included changes of multiple amino acids, carbohydrates (glucose and glycogen), energy metabolism products (ATP and creatinine), and other metabolites (choline and phosphocholine) after ammonia exposure, indicating that ammonia mainly caused disturbance in energy metabolism and amino acids metabolism. The two-dimensional electrophoresis-based proteomic study identified 23 altered proteins, which were involved in nervous system, locomotor system, cytoskeleton assembly, immune stress, oxidative stress, and signal transduction of apoptosis. These results suggested that ammonia not only induced oxidative stress, immune stress, cell injury and apoptosis but also affected the motor ability and central nervous system in marine medaka. It is the first time that metabolomic and proteomic approaches were integrated to elucidate ammonia toxicity in marine fishes. This study is of great value in better understanding the mechanisms of ammonia toxicity in marine fishes and in practical aspects of aquaculture.
Department[Zhu, Limei; Gao, Na; Wang, Ruifang; Zhang, Li] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Gao, Na] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Subject AreaEnvironmental Sciences & Ecology ; Toxicology
WOS KeywordProteomics ; Metabolomics ; Ammonia ; Marine medaka ; Immune stress ; Oxidative stress
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16978
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorzhangli@scsio.ac.cn
Recommended Citation
GB/T 7714
Zhu, LM,Gao, N,Wang, RF,et al. Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure[J]. ECOTOXICOLOGY,2018,27(3):267-277.
APA Zhu, LM,Gao, N,Wang, RF,Zhang, L,&zhangli@scsio.ac.cn.(2018).Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure.ECOTOXICOLOGY,27(3),267-277.
MLA Zhu, LM,et al."Proteomic and metabolomic analysis of marine medaka (Oryzias melastigma) after acute ammonia exposure".ECOTOXICOLOGY 27.3(2018):267-277.
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