Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate
Gong, YH; Ma, SM; Wang, YF; Xu, YK; Sun, AJ; Zhang, Y; Hu, YF; yunfeng.hu@scsio.ac.cn
2018
发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
卷号36期号:2页码:473-482
摘要A novel esterase EstC10 from Bacillus sp. CX01 isolated from the deep sea of the Western Pacific Ocean and the functionalities of EstC10 was characterized. At present, the reports about the kinetic resolution of racemic methyl 2-chloropropionate were quite rare. So we developed deep-sea microbial esterase EstC10 as a novel biocatalyst in the kinetic resolution of racemic methyl 2-chloropropionate and generate (R)-methyl 2-chloropropionate with high enantiomeric excess (> 99%) after the optimization of process parameters such as pH, temperature, organic co-solvents, surfactants, substrate concentration and reaction time. Notably, the optimal substrate concentration (80 mmol/L) of esterase EstC10 was higher than the kinetic resolution of another esterase, Est12-7 (50 mmol/L). The novel microbial esterase EstC10 identified from the deep sea was a promising green biocatalyst in the generation of (R)-methyl 2-chloropropionate as well of many other valuable chiral chemicals in industry.
部门归属[Sun Aijun; Zhang Yun; Hu Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Sun Aijun; Zhang Yun; Hu Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Gong Yanhui; Ma Sanmei; Wang Yongfei] Jinan Univ, Dept Biotechnol, Guangzhou 510632, Guangdong, Peoples R China; [Xu Yongkai] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Jinan 250014, Shandong, Peoples R China; [Hu Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China
学科领域Marine & Freshwater Biology ; Oceanography
关键词[WOS]biocatalysis ; deep-sea microorganisms ; novel esterase ; kinetic resolution ; (R)-methyl 2-chloropropionate
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/16986
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yunfeng.hu@scsio.ac.cn
推荐引用方式
GB/T 7714
Gong, YH,Ma, SM,Wang, YF,et al. Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):473-482.
APA Gong, YH.,Ma, SM.,Wang, YF.,Xu, YK.,Sun, AJ.,...&yunfeng.hu@scsio.ac.cn.(2018).Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),473-482.
MLA Gong, YH,et al."Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):473-482.
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