SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate
Gong, YH; Ma, SM; Wang, YF; Xu, YK; Sun, AJ; Zhang, Y; Hu, YF; yunfeng.hu@scsio.ac.cn
2018
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
Volume36Issue:2Pages:473-482
AbstractA novel esterase EstC10 from Bacillus sp. CX01 isolated from the deep sea of the Western Pacific Ocean and the functionalities of EstC10 was characterized. At present, the reports about the kinetic resolution of racemic methyl 2-chloropropionate were quite rare. So we developed deep-sea microbial esterase EstC10 as a novel biocatalyst in the kinetic resolution of racemic methyl 2-chloropropionate and generate (R)-methyl 2-chloropropionate with high enantiomeric excess (> 99%) after the optimization of process parameters such as pH, temperature, organic co-solvents, surfactants, substrate concentration and reaction time. Notably, the optimal substrate concentration (80 mmol/L) of esterase EstC10 was higher than the kinetic resolution of another esterase, Est12-7 (50 mmol/L). The novel microbial esterase EstC10 identified from the deep sea was a promising green biocatalyst in the generation of (R)-methyl 2-chloropropionate as well of many other valuable chiral chemicals in industry.
Department[Sun Aijun; Zhang Yun; Hu Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Sun Aijun; Zhang Yun; Hu Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Gong Yanhui; Ma Sanmei; Wang Yongfei] Jinan Univ, Dept Biotechnol, Guangzhou 510632, Guangdong, Peoples R China; [Xu Yongkai] Shandong Univ Tradit Chinese Med, Affiliated Hosp, Jinan 250014, Shandong, Peoples R China; [Hu Yunfeng] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China
Subject AreaMarine & Freshwater Biology ; Oceanography
WOS Keywordbiocatalysis ; deep-sea microorganisms ; novel esterase ; kinetic resolution ; (R)-methyl 2-chloropropionate
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16986
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authoryunfeng.hu@scsio.ac.cn
Recommended Citation
GB/T 7714
Gong, YH,Ma, SM,Wang, YF,et al. Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):473-482.
APA Gong, YH.,Ma, SM.,Wang, YF.,Xu, YK.,Sun, AJ.,...&yunfeng.hu@scsio.ac.cn.(2018).Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),473-482.
MLA Gong, YH,et al."Characterization of a novel deep-sea microbial esterase EstC10 and its use in the generation of (R)-methyl2-chloropropionate".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):473-482.
Files in This Item:
File Name/Size DocType Version Access License
Characterization of (487KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.