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Type II toxin/antitoxin system ParE(SO)/CopA(SO) stabilizes prophage CP4So in Shewanella oneidensis
Yao, JY; Guo, YX; Wang, PX; Zeng, ZS; Li, BY; Tang, KH; Liu, XX; Wang, XX; xxwang@scsio.ac.cn
2018
Source PublicationENVIRONMENTAL MICROBIOLOGY
Volume20Issue:3Pages:1224-1239
AbstractToxin/antitoxin (TA) loci are commonly found in mobile genetic elements such as plasmids and prophages. However, the physiological functions of these TA loci in prophages and cross-regulation among these TA loci remain largely unexplored. Here, we characterized a newly discovered type II TA pair, ParE(SO)/CopA(SO), in the CP4So prophage in Shewanella oneidensis. We demonstrated that ParE(SO)/CopA(SO) plays a critical role in the maintenance of CP4So in host cells after its excision. The toxin ParE(SO) inhibited cell growth, resulting in filamentous growth and eventually cell death. The antitoxin CopA(SO) neutralized the toxicity of ParE(SO) through direct protein-protein interactions and repressed transcription of the TA operon by binding to a DNA motif in the promoter region containing two inverted repeats [5-GTANTAC (N)(3) GTANTAC>-3]. CopA(SO) also repressed transcription of another TA system PemK(SO)/PemI(SO) in megaplasmid pMR-1 of S. oneidensis through binding to a highly similar DNA motif in its promoter region. CopA(SO) homologs are widely spread in Shewanella and other Proteobacteria, either as a component of a TA pair or as orphan antitoxins. Our study thus illustrated the cross-regulation of the TA systems in different mobile genetic elements and expanded our understanding of the physiological function of TA systems.
Department[Yao, Jianyun; Guo, Yunxue; Wang, Pengxia; Zeng, Zhenshun; Li, Baiyuan; Tang, Kaihao; Liu, Xiaoxiao; Wang, Xiaoxue] Chinese Acad Sci, South China Sea Inst Oceanol, RNAM Ctr Marine Microbiol, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou, Guangdong, Peoples R China; [Yao, Jianyun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Subject AreaMicrobiology
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16990
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorxxwang@scsio.ac.cn
Recommended Citation
GB/T 7714
Yao, JY,Guo, YX,Wang, PX,et al. Type II toxin/antitoxin system ParE(SO)/CopA(SO) stabilizes prophage CP4So in Shewanella oneidensis[J]. ENVIRONMENTAL MICROBIOLOGY,2018,20(3):1224-1239.
APA Yao, JY.,Guo, YX.,Wang, PX.,Zeng, ZS.,Li, BY.,...&xxwang@scsio.ac.cn.(2018).Type II toxin/antitoxin system ParE(SO)/CopA(SO) stabilizes prophage CP4So in Shewanella oneidensis.ENVIRONMENTAL MICROBIOLOGY,20(3),1224-1239.
MLA Yao, JY,et al."Type II toxin/antitoxin system ParE(SO)/CopA(SO) stabilizes prophage CP4So in Shewanella oneidensis".ENVIRONMENTAL MICROBIOLOGY 20.3(2018):1224-1239.
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