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Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)
Liang, YS; Ding, X; Yu, X; Wang, Y; Zhou, Y; He, JA; Shi, Y; Zhang, Y; Lin, HR; Lu, DQ; ludanqi@mail.sysu.edu.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume74Pages:309-317
AbstractToll-like receptors (TLRs) are one of the most important innate immune receptors, which recognize various pathogen-associated molecular patterns and activate the downstream immune response. Mouse TLR13 has been found to recognize a highly conserved sequence from bacterial or viral RNA and activate the myeloid differentiation primary response gene 88-dependent signaling response. The function of teleost tlr13 is still not fully understood, especially its relationship with bacterial RNA. In our study, we identified and characterized a tlr13 from Epinephelus coioides (orange-spotted grouper). The full-length cDNA of Eco. tlr13 contained a 2844 bp open reading frame, encoding 947 amino acids. The polypeptide was constitutive of a signal peptide, 13 leucine-rich repeats domains, a C-terminal leucine-rich repeats, a transmembrane domain and a conserved Toll/interleukin (IL)-1 receptor domain, indicating that Eco. Tlr13 exhibited a typical TLR structure. Multiple alignments showed that the Toll/IL-1 receptor domain of Eco. Tlr13 was identical with other homologues, and the phylogenetic tree suggested that Eco. Tlr13 was clustered with other TLR13s and had the closest relationship with predicted Lates calcarifer (sea bass) Tlr13. Subcellular localization analysis revealed that Eco. Tlr13 colocalized with the endoplasmic reticulum and early endosome. Moreover, Eco. tlr13 was broadly observed in all tested tissues with the relatively high expressions in the brain and immune-related tissues. After challenged with 19-mer Staphylococcus aureus 23S ribosomal RNA-derived oligoribonucleotide (ORN Sa19), the expression of Eco. tlr13 was significantly up-regulated in grouper spleen cells. Also, the luciferase assay further revealed that with the overexpression of Eco. Tlr13 in human embryonic kidney 293T cells, ORN Sa19 activated the promoter activity of interferon-beta in a dose-dependent pattern. These results indicate that Eco. tlr13 may involve in the recognition of bacterial RNA.
Department[Liang, Yaosi; Ding, Xu; Yu, Xue; He, Jianan; Zhang, Yong; Lin, Haoran; Lu, Danqi] Sun Yat Sen Univ, Inst Aquat Econ Anim, State Key Lab Riocontrol, Guangzhou, Guangdong, Peoples R China; [Liang, Yaosi; Ding, Xu; Yu, Xue; He, Jianan; Zhang, Yong; Lin, Haoran; Lu, Danqi] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Sch Life Sci, Guangzhou, Guangdong, Peoples R China; [Wang, Yu; Lin, Haoran] Sun Yat Sen Univ, Collaborat Innovat Ctr, South China Sea Resource Exploitat & Protect, Guangzhou, Guangdong, Peoples R China; [Zhou, Ying; Lin, Haoran] Hainan Univ, Ocean Coll, Haikou, Hainan, Peoples R China; [Shi, Yu] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordEpinephelus coioides ; tlr13 ; Subcellular distribution ; ORN Sa19
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16991
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorludanqi@mail.sysu.edu.cn
Recommended Citation
GB/T 7714
Liang, YS,Ding, X,Yu, X,et al. Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:309-317.
APA Liang, YS.,Ding, X.,Yu, X.,Wang, Y.,Zhou, Y.,...&ludanqi@mail.sysu.edu.cn.(2018).Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,74,309-317.
MLA Liang, YS,et al."Identification and functional characterization of Toll-like receptor 13 from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 74(2018):309-317.
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