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Molecular cloning and characterization of FADD from the orange-spotted grouper (Epinephelus coioides)
Zhang, X; Zang, SQ; Li, C; Wei, JG; Qin, QW; weijg@scau.edu.cn; qinqw@scau.edu.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume74Pages:517-529
AbstractFas-associated protein with death domain (FADD) is the key adaptor protein that transmits apoptotic signals mediated by the main death receptors. Besides being an essential instrument in cell death, FADD is also implicated in proliferation, cell cycle progression, tumor development, inflammation, innate immunity, and autophagy. In the present study, a FADD homologue (EcFADD) from the orange-spotted grouper (Epinephelus coioides) was cloned and its possible role in fish immunity was analyzed. The full length cDNA of EcFADD contains 808 base pairs (bp), including a 573 bp open reading frame that encodes a 190 amino acid protein with a predicted molecular mass of 21.81 kDa. Quantitative real-time polymerase chain reaction analysis indicated that EcFADD was distributed in all examined tissues. The expression of EcFADD in the spleen of E. coioides was differentially up-regulated when challenged with Singapore grouper iridovirus (SGIV) or poly-inosine-polycytidylic acid(poly[I:C]). EcFADD was abundantly distributed in both the cytoplasm and nucleus in grouper spleen (GS) and fathead minnow (FHM) epithelial cells. Over-expression of EcFADD inhibited SGIV infection and replication and SGIV-induced apoptosis. To achieve antiviral and anti-apoptosis activities, FADD promoted the activation of interferon-stimulated response element (ISRE) and type I interferon (IFN) genes in the antiviral IFN signaling pathway and inhibited activation of apoptosis-related transcription factors p53. Our results not only characterize FADD but also reveal new immune functions and the molecular mechanisms by which FADD responds to virus infection and virus-induced apoptosis.
Department[Zhang, Xin; Li, Chen; Wei, Jingguang; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Zang, Shaoqing] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Zang, Shaoqing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zang, Shaoqing] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordEpinephelus coioides ; FADD ; SGIV ; Cellular localization ; Apoptosis
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/16992
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorweijg@scau.edu.cn; qinqw@scau.edu.cn
Recommended Citation
GB/T 7714
Zhang, X,Zang, SQ,Li, C,et al. Molecular cloning and characterization of FADD from the orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:517-529.
APA Zhang, X.,Zang, SQ.,Li, C.,Wei, JG.,Qin, QW.,...&qinqw@scau.edu.cn.(2018).Molecular cloning and characterization of FADD from the orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,74,517-529.
MLA Zhang, X,et al."Molecular cloning and characterization of FADD from the orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 74(2018):517-529.
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