TBK1 from orange-spotted grouper exerts antiviral activity against fish viruses and regulates interferon response
Hu, Y; Huang, YH; Liu, JX; Zhang, JC; Qin, QW; Huang, XH; qinqw@scsio.ac.cn; huangxh@scsio.ac.cn
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号73页码:92-99
摘要TANK-binding kinase-1 (TBK1) has been well studied in mammals because of its importance in type I interferon induction in antiviral immunity. However, the roles of fish TBK1 in virus infection still remained largely uncertain. In the current study, a TBK1 homolog from orange-spotted grouper (Epinephelus coioides) (EcTBK1) was cloned and its roles in fish viral infections were investigated. Sequence analysis showed that EcTBK1 encoded a 723-amino acid peptide which shared 98% and 73% identity to large yellow croaker (Larimichthys crocea) and human (homo sapiens), respectively. Multiple sequence alignments indicated that EcTBK1 contained conserved domains, including N-terminal kinase domain (KD), the middle ubiquitin-like domain (ULD) and C-terminal coiled-coil (CC) domains. The tissue distribution profiles demonstrated that EcTBK1 gene was constitutively expressed in all examined tissues, with predominant expression in intestine. Temporal expression analysis in vitro showed that the expression levels of EcTBK1 were significantly up-regulated in response to both red-spotted grouper nervous necrosis virus (RGNNV) and Singapore grouper iridovirus (SGIV) infection, suggested that EcTBK1 might exert crucial roles in fish virus infection. Subcellular localization indicated that EcTBK1 expression was primarily in the cytoplasm in GS cells. The ectopic expression of EcTBK1 significantly inhibited both SGIV and RGNNV replication. Furthermore, EcTBK1 overexpression significantly increased the expression levels of interferon related cytokines and pro-inflammatory factors. In addition, the overexpression of EcTBK1 increased the IRF3- and IRF7-regulated interferon promoter ISRE and IFN activity, and the regulatory effect on interferon immune response were dependent on its kinase domain. Together, we speculated that grouper TBK1 exerted antiviral activity against iridovirus and nodavirus via regulating the interferon immune and inflammatory response.
部门归属[Hu, Yin; Liu, Jiaxin; Zhang, Jingcheng; Huang, Xiaohong] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Huang, Youhua; Qin, Qiwei; Huang, Xiaohong] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Hu, Yin; Liu, Jiaxin; Zhang, Jingcheng; Huang, Xiaohong] Univ Chinese Acad Sci, Beijing, Peoples R China; [Hu, Yin; Liu, Jiaxin; Zhang, Jingcheng; Huang, Xiaohong] Chinese Acad Sci, Guangdong Prov Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]Grouper ; TBK1 ; Iridovirus ; Nodavirus ; Antiviral activity ; Interferon
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17005
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn; huangxh@scsio.ac.cn
推荐引用方式
GB/T 7714
Hu, Y,Huang, YH,Liu, JX,et al. TBK1 from orange-spotted grouper exerts antiviral activity against fish viruses and regulates interferon response[J]. FISH & SHELLFISH IMMUNOLOGY,2018,73:92-99.
APA Hu, Y.,Huang, YH.,Liu, JX.,Zhang, JC.,Qin, QW.,...&huangxh@scsio.ac.cn.(2018).TBK1 from orange-spotted grouper exerts antiviral activity against fish viruses and regulates interferon response.FISH & SHELLFISH IMMUNOLOGY,73,92-99.
MLA Hu, Y,et al."TBK1 from orange-spotted grouper exerts antiviral activity against fish viruses and regulates interferon response".FISH & SHELLFISH IMMUNOLOGY 73(2018):92-99.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
TBK1 from orange-spo(2523KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。