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A new L-type lectin (LvLTLC1) from the shrimp Litopenaeus vannamei facilitates the clearance of Vibrio harveyi
Tian, YS; Chen, T; Huang, W; Luo, P; Huo, D; Yun, L; Hu, CQ; Cai, YM; luopeng@scsio.ac.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume73Pages:185-191
AbstractAccording to structures and functions of lectins found in shrimp, they are classified into seven types, namely, L-type, C-type, P-type, M-type, galectins, fibrinogen-like domain lectins, and calnexin/calreticulin. Until now, the researches of shrimp lectins are mainly focused on C-type lectins. In this study, we identified a new L-type lectin, designated as LvLTLC1, from the shrimp Litopenaeus vannamei. The cDNA of LvLTLC1 is 1184 bp with an open reading frame of 990 bp encoding a protein of 329 amino acids. The LvLTLC1 protein contained a putative signal peptide, an L-type lectin-like domain, and a transmembrane helix region. Phylogenetic analysis showed that LvLTLC1 belonged to VIP36-like family. LvLTLC1 was expressed in all examined tissues but had higher expression level in gills and hepatopancreas than other tissues. LvLTLC1 expression was up-regulated after immune challenge by Vibrio harveyi and lipopolysaccharide. The recombinant LvLTLC1 agglutinated Gram-positive (Staphylococcus aureus) and Gram-negative bacteria (V. harveyi, V. parahaemolyticus, V. alginolyticus, V. cholerae, V. vulnificus, Pseudomonas aeruginosa, P. fluorescens) in a calcium-independent manner. Recombinant LvLTLC1 exerted the ability of enhancing the clearance of V. harveyi injected in shrimp. Our results indicated that LvLTLC1 functions in anti-pathogen innate immunity of shrimp.
Department[Tian, Yushun; Chen, Ting; Huang, Wen; Luo, Peng; Huo, Da; Yun, Long; Hu, Chaoqun; Cai, Yiming] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 501301, Guangdong, Peoples R China; [Tian, Yushun; Huo, Da; Yun, Long] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Ting; Huang, Wen; Luo, Peng; Hu, Chaoqun; Cai, Yiming] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510301, Guangdong, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordLitopenaeus vannamei ; L-type lectin ; Calcium-independent ; Innate immunity
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17006
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorluopeng@scsio.ac.cn
Recommended Citation
GB/T 7714
Tian, YS,Chen, T,Huang, W,et al. A new L-type lectin (LvLTLC1) from the shrimp Litopenaeus vannamei facilitates the clearance of Vibrio harveyi[J]. FISH & SHELLFISH IMMUNOLOGY,2018,73:185-191.
APA Tian, YS.,Chen, T.,Huang, W.,Luo, P.,Huo, D.,...&luopeng@scsio.ac.cn.(2018).A new L-type lectin (LvLTLC1) from the shrimp Litopenaeus vannamei facilitates the clearance of Vibrio harveyi.FISH & SHELLFISH IMMUNOLOGY,73,185-191.
MLA Tian, YS,et al."A new L-type lectin (LvLTLC1) from the shrimp Litopenaeus vannamei facilitates the clearance of Vibrio harveyi".FISH & SHELLFISH IMMUNOLOGY 73(2018):185-191.
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