SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata
Zhang, H; Huang, XD; Shi, Y; Liu, WG; He, MX; hmx2@scsio.ac.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume73Pages:279-287
AbstractThe mitogen-activated protein kinase kinase 4 (MKK4) is a key component of the c-Jun N-terminal kinase (JNK) signaling pathway and regulates multiple cellular activities. However, little is known about the roles of this kinase in pearl oyster. In this study, we identified an MKK4 homologue in Pinctada fucata by using a transcriptome database. Sequence analysis and protein structure prediction showed that PfMKK4 is highly conserved to MKK4 from other vertebrate and invertebrate species. Phylogenetic analysis revealed that PfMKK4 has the closest relationship with that from Crassostrea gigas. QPCR was used to investigate expression profiles in different healthy adult tissues and developmental stages of P. fucata. We found that PfMKK4 was ubiquitously expressed in all tissues and developmental stages examined except for in D-shaped larvae. Gene expression analysis suggested that PfMKK4 is involved in the response to the nucleus insertion operation. Lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid [poly(I:C)] stimulation in vivo reduced PfMKK4 mRNA expression at 6 h, 48 h and 48 h, 72 h, respectively. LPS and poly(I:C) induced PfMKK4 phosphorylation in a primary mantle cell culture. These results contribute to better understanding of the potential role played by PfMKK4 in protecting the pearl oyster from injury caused by grafting or disease.
Department[Zhang, Hua; Huang, Xiande; Shi, Yu; Liu, Wenguang; He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Hua] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordPinctada fucata ; PfMKK4 ; Gene expression ; Graft ; Immunity
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17007
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhmx2@scsio.ac.cn
Recommended Citation
GB/T 7714
Zhang, H,Huang, XD,Shi, Y,et al. Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata[J]. FISH & SHELLFISH IMMUNOLOGY,2018,73:279-287.
APA Zhang, H,Huang, XD,Shi, Y,Liu, WG,He, MX,&hmx2@scsio.ac.cn.(2018).Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata.FISH & SHELLFISH IMMUNOLOGY,73,279-287.
MLA Zhang, H,et al."Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata".FISH & SHELLFISH IMMUNOLOGY 73(2018):279-287.
Files in This Item:
File Name/Size DocType Version Access License
Identification and a(2158KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.