Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata
Zhang, H; Huang, XD; Shi, Y; Liu, WG; He, MX; hmx2@scsio.ac.cn
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号73页码:279-287
摘要The mitogen-activated protein kinase kinase 4 (MKK4) is a key component of the c-Jun N-terminal kinase (JNK) signaling pathway and regulates multiple cellular activities. However, little is known about the roles of this kinase in pearl oyster. In this study, we identified an MKK4 homologue in Pinctada fucata by using a transcriptome database. Sequence analysis and protein structure prediction showed that PfMKK4 is highly conserved to MKK4 from other vertebrate and invertebrate species. Phylogenetic analysis revealed that PfMKK4 has the closest relationship with that from Crassostrea gigas. QPCR was used to investigate expression profiles in different healthy adult tissues and developmental stages of P. fucata. We found that PfMKK4 was ubiquitously expressed in all tissues and developmental stages examined except for in D-shaped larvae. Gene expression analysis suggested that PfMKK4 is involved in the response to the nucleus insertion operation. Lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid [poly(I:C)] stimulation in vivo reduced PfMKK4 mRNA expression at 6 h, 48 h and 48 h, 72 h, respectively. LPS and poly(I:C) induced PfMKK4 phosphorylation in a primary mantle cell culture. These results contribute to better understanding of the potential role played by PfMKK4 in protecting the pearl oyster from injury caused by grafting or disease.
部门归属[Zhang, Hua; Huang, Xiande; Shi, Yu; Liu, Wenguang; He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Hua] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]Pinctada fucata ; PfMKK4 ; Gene expression ; Graft ; Immunity
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17007
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx2@scsio.ac.cn
推荐引用方式
GB/T 7714
Zhang, H,Huang, XD,Shi, Y,et al. Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata[J]. FISH & SHELLFISH IMMUNOLOGY,2018,73:279-287.
APA Zhang, H,Huang, XD,Shi, Y,Liu, WG,He, MX,&hmx2@scsio.ac.cn.(2018).Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata.FISH & SHELLFISH IMMUNOLOGY,73,279-287.
MLA Zhang, H,et al."Identification and analysis of an MKK4 homologue in response to the nucleus grafting operation and antigens in the pearl oyster, Pinctada fucata".FISH & SHELLFISH IMMUNOLOGY 73(2018):279-287.
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