Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara
Liu, XF; Wu, YH; Wei, SN; Wang, N; Li, PF; Li, YZ; Zhang, NW; Qin, QW; Chen, SL; chensl@ysfri.ac.cn
2018
发表期刊FISH PHYSIOLOGY AND BIOCHEMISTRY
卷号44期号:1页码:87-93
摘要A novel cell line, Epinephelus moara kidney cell line (EMK), was established from kidneys of kelp grouper E. moara. Cells were cultured at 24 A degrees C in Leibovitz's L-15 medium (L15) supplemented with antibiotics, basic fibroblast growth factor (bFGF), foetal bovine serum (FBS) and 2-mercaptoethanol (2-ME). EMK cells, fibroblastic in morphology, proliferated to 100% confluency in 3-4 days and were subcultured for over 50 passages. The cells could grow from 18 to 30 A degrees C, with optimal growth at 24 A degrees C. Chromosome analysis indicated that the modal chromosome number was 48 in the cells at passage 42. Green fluorescent signals could be observed in EMK cells when the cells were transfected with pEGFP-N3 plasmid. Moreover, a significant cytopathic effect (CPE) was observed in the cells after infection with Singapore grouper iridovirus (SGIV) or nervous necrosis virus (NNV), and viral replication was confirmed by quantitative real-time PCR (qPCR). These results suggested the potential of the EMK cell line for studies of transgene and pathogenesis of SGIV and NNV.
部门归属[Liu, Xiao-Feng; Wu, Ya-Hong; Wang, Na; Li, Yang-Zhen; Zhang, Nian-Wei; Chen, Song-Lin] CAFS, Minist Agr, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Qingdao 266071, Peoples R China; [Liu, Xiao-Feng; Chen, Song-Lin] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China; [Liu, Xiao-Feng] Ocean Univ China, Coll Marine Life, Qingdao 266003, Peoples R China; [Wei, Shi-Na; Li, Peng-Fei; Qin, Qi-Wei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China
学科领域Biochemistry & Molecular Biology ; Fisheries ; Physiology
关键词[WOS]Kidney cell line ; Epinephelus moara ; Transfection ; SGIV ; NNV
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17012
专题中科院海洋生物资源可持续利用重点实验室
通讯作者chensl@ysfri.ac.cn
推荐引用方式
GB/T 7714
Liu, XF,Wu, YH,Wei, SN,et al. Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2018,44(1):87-93.
APA Liu, XF.,Wu, YH.,Wei, SN.,Wang, N.,Li, PF.,...&chensl@ysfri.ac.cn.(2018).Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.FISH PHYSIOLOGY AND BIOCHEMISTRY,44(1),87-93.
MLA Liu, XF,et al."Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara".FISH PHYSIOLOGY AND BIOCHEMISTRY 44.1(2018):87-93.
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