Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa
Wei, FL; Yu, LX; Li, RH; Zhang, X; Zhang, XH; Zhang, Y; Wang, YQ; Wang, HC; Liang, J; Ma, R; Qi, HF; Qin, QW; Zhang, RQ; Zhu, SH; Li, CZ; lichangzhong@qhu.edu.cn
2018
发表期刊ANIMAL REPRODUCTION SCIENCE
卷号188页码:13-20
摘要The endemic naked carp (Gymnocypris przewalskii Kessler, 1876) plays an important role in the maintenance of the distinctive ecological system of Lake Qinghai at 3.2 km altitude on the Qinghai-Tibet Plateau in China. This study aimed to develop a cryopreservation protocol for Gymnocypris przewalskii spermatozoa. Semen was collected from mature individuals during migration and frozen using the liquid nitrogen vapor method. The influence of different cryoprotectants and three extenders on the post-thaw quality of the sperm was analyzed. The highest sperm motility rate and longest motility time after cryopreservation were achieved by combining Ringer's solution with 15% ethylene glycol (P < 0.05). The fertilization rate of this cryopreserved semen was 15.26 +/- 4.54%. This study thus provides a valuable method for the cryopreservation of the sperm of this important endangered fish species.
部门归属[Wei, Fulei; Li, Ruihong; Zhang, Xia; Zhang, Xuehan; Zhang, Ya; Wang, Yuqing; Liang, Jian; Ma, Rui; Zhu, Shihai; Li, Changzhong] Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Qinghai, Peoples R China; [Yu, Luxian; Wang, Hongchao; Qi, Hongfang] Gymnocypris Przewalskii Rescue Ctr, Xining 810016, Qinghai, Peoples R China; [Qin, Qiwei] Chinese Acad Sci, Key Lab Sustainable Utilizat Marine Bioresources, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Rongqing] Tsinghua Univ, Inst Marine Biotechnol, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
学科领域Agriculture ; Reproductive Biology
关键词[WOS]Semen conservation ; Freezing medium ; Naked carp ; Lake Qinghai
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17029
专题中科院海洋生物资源可持续利用重点实验室
通讯作者lichangzhong@qhu.edu.cn
推荐引用方式
GB/T 7714
Wei, FL,Yu, LX,Li, RH,et al. Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa[J]. ANIMAL REPRODUCTION SCIENCE,2018,188:13-20.
APA Wei, FL.,Yu, LX.,Li, RH.,Zhang, X.,Zhang, XH.,...&lichangzhong@qhu.edu.cn.(2018).Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa.ANIMAL REPRODUCTION SCIENCE,188,13-20.
MLA Wei, FL,et al."Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa".ANIMAL REPRODUCTION SCIENCE 188(2018):13-20.
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