SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides
Ni, SW; Yu, YP; Wei, JG; Zhou, LL; Wei, SN; Yan, Y; Huang, XH; Huang, YH; Qin, QW; huangyh@scsio.ac.cn; qinqw@scsio.ac.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume72Pages:9-13
AbstractMicroRNA-146a (miR-146a) has been demonstrated to function as a negative regulator of cellular immune responses against pathogens in mammals, however, little information focused on its functions in lower vertebrates. In this study, we investigated the regulatory roles of orange spotted grouper, Epinephelus coioides miR-146a during red spotted grouper nervous necrosis virus (RGNNV) infection. During RGNNV infection in grouper spleen (GS) cells, the endogenous expression level of miR-146a and tumor necrosis factor receptor-associated factor 6 (TRAF6) significantly increased along with the infection time. Overexpression of miR-146a significantly facilitated viral infection, evidenced by the increased transcription of viral CP and RdRp genes, while miR-146a knockdown by specific inhibitors decreased RGNNV replication. Using pMIR-REPORT Luciferase system, we found that the 3' untranslated region (UTR) of grouper TRAF6 could be specifically targeted by miR-146a. Further studies showed that its downstream target gene pro-inflammatory cytokines, including TNF-alpha, IL-8 and IL-beta, were all significantly decreased in miR-146a mimic transfected cells, but increased in miR-146a inhibitors transfected cells during RGNNV infection. Thus, our results suggested and verified that holding the level of miR-146a exerted crucial roles in RGNNV infection through TRAF6-mediated inflammatory response.
Department[Ni, Songwei; Yu, Yepin; Wei, Jingguang; Zhou, Lingli; Yan, Yang; Huang, Xiaohong; Huang, Youhua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Yu, Yepin; Wei, Jingguang; Wei, Shina; Huang, Xiaohong; Huang, Youhua; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Ni, Songwei; Yu, Yepin; Zhou, Lingli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Qin, Qiwei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordmiR-146a ; RGNNV ; Grouper ; TRAF6 ; Viral infection
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17030
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhuangyh@scsio.ac.cn; qinqw@scsio.ac.cn
Recommended Citation
GB/T 7714
Ni, SW,Yu, YP,Wei, JG,et al. MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:9-13.
APA Ni, SW.,Yu, YP.,Wei, JG.,Zhou, LL.,Wei, SN.,...&qinqw@scsio.ac.cn.(2018).MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,72,9-13.
MLA Ni, SW,et al."MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 72(2018):9-13.
Files in This Item:
File Name/Size DocType Version Access License
MicroRNA-146a promot(549KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.