MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides
Ni, SW; Yu, YP; Wei, JG; Zhou, LL; Wei, SN; Yan, Y; Huang, XH; Huang, YH; Qin, QW; huangyh@scsio.ac.cn; qinqw@scsio.ac.cn
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号72页码:9-13
摘要MicroRNA-146a (miR-146a) has been demonstrated to function as a negative regulator of cellular immune responses against pathogens in mammals, however, little information focused on its functions in lower vertebrates. In this study, we investigated the regulatory roles of orange spotted grouper, Epinephelus coioides miR-146a during red spotted grouper nervous necrosis virus (RGNNV) infection. During RGNNV infection in grouper spleen (GS) cells, the endogenous expression level of miR-146a and tumor necrosis factor receptor-associated factor 6 (TRAF6) significantly increased along with the infection time. Overexpression of miR-146a significantly facilitated viral infection, evidenced by the increased transcription of viral CP and RdRp genes, while miR-146a knockdown by specific inhibitors decreased RGNNV replication. Using pMIR-REPORT Luciferase system, we found that the 3' untranslated region (UTR) of grouper TRAF6 could be specifically targeted by miR-146a. Further studies showed that its downstream target gene pro-inflammatory cytokines, including TNF-alpha, IL-8 and IL-beta, were all significantly decreased in miR-146a mimic transfected cells, but increased in miR-146a inhibitors transfected cells during RGNNV infection. Thus, our results suggested and verified that holding the level of miR-146a exerted crucial roles in RGNNV infection through TRAF6-mediated inflammatory response.
部门归属[Ni, Songwei; Yu, Yepin; Wei, Jingguang; Zhou, Lingli; Yan, Yang; Huang, Xiaohong; Huang, Youhua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China; [Yu, Yepin; Wei, Jingguang; Wei, Shina; Huang, Xiaohong; Huang, Youhua; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Ni, Songwei; Yu, Yepin; Zhou, Lingli] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Qin, Qiwei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]miR-146a ; RGNNV ; Grouper ; TRAF6 ; Viral infection
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17030
专题中科院海洋生物资源可持续利用重点实验室
通讯作者huangyh@scsio.ac.cn; qinqw@scsio.ac.cn
推荐引用方式
GB/T 7714
Ni, SW,Yu, YP,Wei, JG,et al. MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:9-13.
APA Ni, SW.,Yu, YP.,Wei, JG.,Zhou, LL.,Wei, SN.,...&qinqw@scsio.ac.cn.(2018).MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,72,9-13.
MLA Ni, SW,et al."MicroRNA-146a promotes red spotted grouper nervous necrosis virus (RGNNV) replication by targeting TRAF6 in orange spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 72(2018):9-13.
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