The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge
Yan, AF; Ren, CH; Chen, T; Jiang, X; Sun, HY; Huo, D; Hu, CQ; Wen, J; yanaifen@mail3.sysu.edu.cn; rosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; hongyanlucky@scau.edu.cn; 827920154@qq.com; hucq@scsio.ac.cn; jw82123@126.com
2018
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号72页码:124-131
摘要In this study, the first tropical sea cucumber caspase-8 named HLcaspase-8 was identified from Holothuria leucospilota. The full-length cDNA of HLcaspase-8 is 2293 bp in size, containing a 245 bp 5'-untranslated region (UTR), a 521 bp 3'-UTR and a 1527 bp open reading frame (ORF) encoding a protein of 508 amino acids with a deduced molecular weight of 57.47 kDa. Besides the common signatures of caspase family including conserved cysteine active site pentapeptide motif QACQG, P20 domain and P10 domain, HLcaspase-8 also contains a characteristic DED domain. The over-expression of HLcaspase-8 in HEK293T cells showed that HLcaspase-8 protein could induce apoptosis and the apoptosis could be promoted by TNF-alpha, indicating that the apoptosis induced by HLcaspase-8 might also be triggered via a receptor-mediated pathway. Moreover, the expression of HLcaspase-8 in in vitro experiments performed in coelomocytes was significantly up-regulated by lipopolysaccharides (LPS) or polyriboinosinic-polyribocytidylic Acid [poly (I:C)] challenge, suggesting that the sea cucumber caspase-8 might play some important roles in the innate immune defense against bacterial and viral infections.
部门归属[Yan, Aifen] Foshan Univ, Sch Stomatol & Med, Foshan 528000, Peoples R China; [Ren, Chunhua; Chen, Ting; Jiang, Xiao; Huo, Da; Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China; [Ren, Chunhua; Chen, Ting; Hu, Chaoqun] South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China; [Sun, Hongyan] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Wen, Jing] Lingnan Normal Univ, Dept Biol, Zhanjiang 524048, Peoples R China
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]Caspase-8 ; Holothuria leucospilota ; Apoptosis ; Innate immunity
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17032
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yanaifen@mail3.sysu.edu.cn; rosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; hongyanlucky@scau.edu.cn; 827920154@qq.com; hucq@scsio.ac.cn; jw82123@126.com
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Yan, AF,Ren, CH,Chen, T,et al. The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2018,72:124-131.
APA Yan, AF.,Ren, CH.,Chen, T.,Jiang, X.,Sun, HY.,...&jw82123@126.com.(2018).The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge.FISH & SHELLFISH IMMUNOLOGY,72,124-131.
MLA Yan, AF,et al."The first tropical sea cucumber caspase-8 from Holothuria leucospilota: Molecular characterization, involvement of apoptosis and inducible expression by immune challenge".FISH & SHELLFISH IMMUNOLOGY 72(2018):124-131.
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