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Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51
Huang, XC; Chen, C; Ren, CH; Li, YY; Deng, YQ; Yang, YY; Ding, XQ; chen.chang@scsio.ac.cn
2018
Source PublicationBIOFOULING
Volume34Issue:1Pages:1-14
AbstractColanic acid (CA) is a group I extracellular polysaccharide (EPS) that contributes to resistance against adverse environments in many members of the Enterobacteriaceae. In the present study, a genetic locus EPSC putatively involved in CA biosynthesis was identified in Vibrio alginolyticus ZJ-51, which undergoes colony morphology variation between translucent/smooth (ZJ-T) and opaque/rugose (ZJ-O). EPSC in ZJ-T carries 21 ORFs and resembles the CA cluster of Escherichia coli K-12. The deletion of EPSC led to decreased EPS and biofilm formation in both genetic backgrounds but no alternation of lipopolysaccharide. The loss of this locus also changed the colony morphology of ZJ-O on the 2216E plate and reduced the motility of ZJ-T. Compared with ZJ-T, ZJ-O lacks a 10-kb fragment (eps(T)) in EPSC containing homologs of wecA, wzx and wzy that are essential for O-antigen synthesis. However, the deletion or overexpression of eps(T) resulted in no change of colony morphology, biofilm formation or EPS production. This study reported at the first time a genetic locus EPSC that may be involved in colanic acid synthesis in V. alginolyticus ZJ-51, and found that it was related to EPS biosynthesis, biofilm formation, colony morphology and motility, which may shed light on the environmental adaptation of the vibrios.
Department[Huang, Xiaochun; Chen, Chang; Ren, Chunhua; Deng, Yiqin; Yang, Yiying; Ding, Xiongqi] Univ Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China; [Huang, Xiaochun; Chen, Chang; Deng, Yiqin; Yang, Yiying; Ding, Xiongqi] Univ Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou, Guangdong, Peoples R China; [Chen, Chang] Univ Chinese Acad Sci, South China Sea Inst Oceanol, Xisha Deep Sea Marine Environm Observat & Res Stn, Guangzhou, Guangdong, Peoples R China; [Huang, Xiaochun; Deng, Yiqin; Yang, Yiying; Ding, Xiongqi] Univ Chinese Acad Sci, Beijing, Peoples R China; [Li, Yingying] Jinan Univ, Coll Life Sci & Technol, Guangzhou, Guangdong, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS KeywordColanic acid ; EPS ; biofilm formation ; colony phase variation ; Vibrio alginolyticus
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17034
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorchen.chang@scsio.ac.cn
Recommended Citation
GB/T 7714
Huang, XC,Chen, C,Ren, CH,et al. Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51[J]. BIOFOULING,2018,34(1):1-14.
APA Huang, XC.,Chen, C.,Ren, CH.,Li, YY.,Deng, YQ.,...&chen.chang@scsio.ac.cn.(2018).Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51.BIOFOULING,34(1),1-14.
MLA Huang, XC,et al."Identification and characterization of a locus putatively involved in colanic acid biosynthesis in Vibrio alginolyticus ZJ-51".BIOFOULING 34.1(2018):1-14.
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