Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways
Yan, AF; Chen, YF; Chen, S; Li, SS; Zhang, Y; Jia, JR; Yu, H; Liu, L; Liu, F; Hu, CQ; Tang, DS; Chen, T; yanaifen@mail3.sysu.edu.cn; chyfwf@hotmail.com; chenss@connect.hku.hk; lshuish@mail.sysu.edu.cn; lsszy@mail.sysu.edu.cn; jiajr@mail2.sysu.edu.cn; yu71hui@126.com; lian2004@163.com; 1115fang@sina.com; hucq@scsio.ac.cn; tangdsh@163.com; chan1010@scsio.ac.cn
2018
发表期刊INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号18期号:12页码:-
摘要Leptin actions at the pituitary level have been extensively investigated in mammalian species, but remain insufficiently characterized in lower vertebrates, especially in teleost fish. Prolactin (PRL) is a pituitary hormone of central importance to osmoregulation in fish. Using goldfish as a model, we examined the global and brain-pituitary distribution of a leptin receptor (lepR) and examined the relationship between expression of lepR and major pituitary hormones in different pituitary regions. The effects of recombinant goldfish leptin-AI and leptin-AII on PRL mRNA expression in the pituitary were further analysed, and the mechanisms underlying signal transduction for leptin-induced PRL expression were determined by pharmacological approaches. Our results showed that goldfish lepR is abundantly expressed in the brain-pituitary regions, with highly overlapping PRL transcripts within the pituitary. Recombinant goldfish leptin-AI and leptin-AII proteins could stimulate PRL mRNA expression in dose-and time-dependent manners in the goldfish pituitary, by both intraperitoneal injection and primary cell incubation approaches. Moreover, the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK, and MEK1/2/ERK1/2-but not JAK2/STAT 1, 3 and 5 cascades-were involved in leptin-induced PRL mRNA expression in the goldfish pituitary.
部门归属[Yan, Aifen; Chen, Yanfeng; Yu, Hui; Liu, Lian; Liu, Fang; Tang, Dongsheng] Foshan Univ, Foshan 528000, Peoples R China; [Chen, Shuang] BGI, Shenzhen 518083, Peoples R China; [Li, Shuisheng; Zhang, Yong; Jia, Jirong] Sun Yat Sen Univ, Inst Aquat Econ Anim, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China; [Li, Shuisheng; Zhang, Yong; Jia, Jirong] Sun Yat Sen Univ, Guangdong Prov Key Lab Aquat Econ Anim, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China; [Hu, Chaoqun; Chen, Ting] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, Guangzhou 510301, Guangdong, Peoples R China
学科领域Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]goldfish ; leptin ; prolactin ; pituitary ; expression regulation ; signal pathway
资助项目LMB
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/17039
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yanaifen@mail3.sysu.edu.cn; chyfwf@hotmail.com; chenss@connect.hku.hk; lshuish@mail.sysu.edu.cn; lsszy@mail.sysu.edu.cn; jiajr@mail2.sysu.edu.cn; yu71hui@126.com; lian2004@163.com; 1115fang@sina.com; hucq@scsio.ac.cn; tangdsh@163.com; chan1010@scsio.ac.cn
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Yan, AF,Chen, YF,Chen, S,et al. Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2018,18(12):-.
APA Yan, AF.,Chen, YF.,Chen, S.,Li, SS.,Zhang, Y.,...&chan1010@scsio.ac.cn.(2018).Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,18(12),-.
MLA Yan, AF,et al."Leptin Stimulates Prolactin mRNA Expression in the Goldfish Pituitary through a Combination of the PI3K/Akt/mTOR, MKK3/6/p(38)MAPK and MEK1/2/ERK1/2 Signalling Pathways".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 18.12(2018):-.
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