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A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges
Qu, FF; Xiang, ZM; Zhou, YL; Qin, YP; qufufa@163.com
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume71Pages:105-115
AbstractTumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) is a member of the TRAF superfamily that acted as a key signal transduction protein and has been implicated in inflammatory and apoptosis processes in mammals. However, identification of TRAF2s in invertebrates is very limited and its function, in particular that under immune challenges, is still unknown. In this report, a molluscan TRAF2 gene (referred to as AwTRAF2) was cloned and characterized from the freshwater bivalve, Anodonta woodiana. The open reading frame (ORF) of AwTRAF2 was 1683 bp in length, which encoded a putative 560 amino acid-protein. The deduced AwTRAF2 sequence shared similar structural characteristics and close evolutionary relationship with mollusk TRAF2s. The tissue-specific expression analysis revealed that AwTRAF2 mRNA was broadly expressed in all tested tissues, with high expression in gill and hepatopancreas. In addition, in vivo injection experiments directly showed that AwTRAF2 mRNA levels in hepatopancreas were significantly up-regulated in response to bacterial pathogen (Vibrio alginolyticus and Staphylococcus aureus) and PAMPs (Lipopolysaccharides and Peptidoglycan) challenges. Moreover, fluorescence microscopy observations revealed that AwTRAF2 was mainly located in cytoplasm of HEK293T cells and its overexpression significantly increased the transcriptional activities of the NF-kappa B-Luc reporter gene in HEK293T cells. Taken together, this study provided the experimental evidence of the presence of a functional TRAF2 in freshwater bivalves, which revealed its involvement in host response to immune challenges in A. woodiana. (C) 2017 Elsevier Ltd. All rights reserved.
Department[Qu, Fufa] Changsha Univ, Dept Biol & Environm Engn, Changsha 410022, Hunan, Peoples R China; [Qu, Fufa; Xiang, Zhiming; Zhou, Yingli; Qin, Yanping] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Fufa; Xiang, Zhiming; Zhou, Yingli; Qin, Yanping] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Qu, Fufa; Xiang, Zhiming; Zhou, Yingli; Qin, Yanping] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510275, Guangdong, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordTNF receptor-associated factor 2 ; Molecular characterization ; Immune challenge ; NF-kappa B ; Anodonta woodiana
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17041
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqufufa@163.com
Recommended Citation
GB/T 7714
Qu, FF,Xiang, ZM,Zhou, YL,et al. A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges[J]. FISH & SHELLFISH IMMUNOLOGY,2018,71:105-115.
APA Qu, FF,Xiang, ZM,Zhou, YL,Qin, YP,&qufufa@163.com.(2018).A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges.FISH & SHELLFISH IMMUNOLOGY,71,105-115.
MLA Qu, FF,et al."A molluscan TNF receptor-associated factor 2 (TRAF2) was involved in host defense against immune challenges".FISH & SHELLFISH IMMUNOLOGY 71(2018):105-115.
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