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Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides)
Wei, JG; Zhang, X; Zang, SQ; Qin, QW; qinqw@scau.edu.cn
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume71Pages:295-304
AbstractAntiviral immune responses are triggered by the innate immune recognition of viral infection. Toll/interleukin-1 receptor (TIR) domain containing adapter inducing interferon-beta (TRIF) is an adapter in responding to activation of Toll-like receptors, which provides early clearance of viral pathogens. Our study focuses on the functional characterization of grouper TRIF (EcTRIF) based on the comparison of its sequence and functional evolution from grouper fish to mammals. The results show that the open reading frame of EcTRIF encoded a protein of 580 amino acids. Real-time PCR analysis indicates that EcTRIF was constitutively expressed in all the analyzed tissues in healthy grouper. EcTRIF was significantly induced in spleen post-LPS and poly (I:C) stimulation. Fluorescence microscopy shows that EcTRIF is colocalized with a Golgi apparatus marker, implying its unique subcellular localization in the Golgi apparatus. Luciferase reporter assays confirmed that EcTRIF was able to activate the IFN and NF-kappa B promoter. Overexpression of EcTRIF in grouper brain cells inhibited the replication of red-spotted grouper nervous necrosis virus (RGNNV). These results indicate that EcTRIF plays an important role in modulating antiviral innate immune responses. Our results have applications in functional studies on TRIF in teleost fish and immune evolution. (C) 2017 Elsevier Ltd. All rights reserved.
Department[Wei, Jingguang; Zhang, Xin; Qin, Qiwei] South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China; [Zang, Shaoqing] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Zang, Shaoqing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zang, Shaoqing] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China; [Qin, Qiwei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS KeywordEpinephelus coioides ; TRIF ; Cellular localization ; RGNNV ; Antiviral immunity
Funding ProjectLMB
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17043
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorqinqw@scau.edu.cn
Recommended Citation
GB/T 7714
Wei, JG,Zhang, X,Zang, SQ,et al. Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2018,71:295-304.
APA Wei, JG,Zhang, X,Zang, SQ,Qin, QW,&qinqw@scau.edu.cn.(2018).Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,71,295-304.
MLA Wei, JG,et al."Expression and functional characterization of TRIF in orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 71(2018):295-304.
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