SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Molecular characterization and functional analysis of MyD88 from the tropical sea cucumber, Holothuria leucospilota
Zhao, Lin; Jiang, Xiao1; Chen, Ting1; Sun, Hongyan2; Ren, Chunhua1
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume83Pages:1
AbstractIn this study, a myeloid differentiation factor 88 (MyD88) named as HLMyD88 was identified from the sea cucumber Holothuria leucospilota. The full-length cDNA of HLMyD88 is 4797 bp in size, containing a 227 bp 5'-untranslated region (UTR), a 3721 bp 3'-UTR and an 849 bp open reading frame (ORF) encoding a protein of 282 amino acids with a deduced molecular weight of 32.25 kDa HLMyD88 contains an N-terminal death domain and a C-terminal Toll/interluekin-1 receptor (TIR) domain with three highly conserved sequence motifs named as Box 1, Box 2 and Box 3. The results of luciferase reporter assay showed that over-expressed HLMyD88 in HEK293T cells could activate the transcription factors nuclear factor-kappa B (NF-kappa B) and activator protein 1 (AP-1). Additionally, the secretion of proinflammatory cytokines IL-1 beta and TNF-alpha in the HEK293T cells was increased by over-expressed HLMyD88, indicating the potential role of HLMyD88 in the innate immunity of sea cucumber. Moreover, we further confirmed that over-expressed HLMyD88 could also induce apoptosis.
DepartmentLMB
KeywordMyD88 Holothuria leucospilota Nuclear factor-kappa B Activator protein 1 Proinflammatory cytokines Apoptosis Innate immunity
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17599
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Biotechnol Candidate Drug, Guangzhou 510006, Guangdong, Peoples R China
2.Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangdong Prov Key Lab Appl Marine Biol LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
3.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Lin,Jiang, Xiao,Chen, Ting,et al. Molecular characterization and functional analysis of MyD88 from the tropical sea cucumber, Holothuria leucospilota[J]. FISH & SHELLFISH IMMUNOLOGY,2018,83:1, 7.
APA Zhao, Lin,Jiang, Xiao,Chen, Ting,Sun, Hongyan,&Ren, Chunhua.(2018).Molecular characterization and functional analysis of MyD88 from the tropical sea cucumber, Holothuria leucospilota.FISH & SHELLFISH IMMUNOLOGY,83,1.
MLA Zhao, Lin,et al."Molecular characterization and functional analysis of MyD88 from the tropical sea cucumber, Holothuria leucospilota".FISH & SHELLFISH IMMUNOLOGY 83(2018):1.
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