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Remarkable sequence polymorphisms in 18S rDNA of Pleuronichthys cornutus (Pleuronectiformes: Pleuronectidae)
Yang, Min1; Kong, Xiaoyu; Shi, Wei; Gong, Li2; Luo, Hairong1; Wu, Baosheng1
Source PublicationGENE
AbstractHighly conserved 18S rDNA sequences encode ribosomal RNA and evolve in a concerted manner. In this study, 178 sequences of 18S rDNA from the ridge-eyed flounder, Pleuronichthys cornutus, were analyzed. The total sequences yielded five distinguishable types of 18S rDNA A, B, R, S, and L-that were defined based on sequence alignments, clone clustering, and recombination detection. The length of 176 clones ranged from 1838 bp to 1846 bp, with one particularly short clone reaching only 1466 bp and one long clone reaching up to1869 bp. As per current criteria for pseudogene inference, Type S was inferred to be a pseudogene due to its truncated length (380 bp) and low minimum free energy (- 536.9 kcal-mol(-1)). Type L had a unique 20-bp insertion and was also predicted to be as a pseudogene. Types A and B showed 31 differential sites, which in Type A was consistent with 18S rDNA sequences found in six other flounders, while Type B and recombinant Type R were not. Maximum K2P genetic distances were calculated within Type B (0.0232) and R (0.0313); these were much higher than that of Type A (0.0093) or between the six flounders (0.011). Only Type A was detected by reverse transcription experiments and linked to functional ITS1 and 5.8S fragments; Types B and R were linked to both functional and pseudo genes. Accordingly, Type A is likely the functional gene, and Types B and R are probable pseudogenes. The heterogeneity of the five types revealed that 18S rDNA sequences evolved in a non-concerted evolution manner in P. comutus. Furthermore, our results suggest that more features should be used to identify functional or pseudo genes.
Keyword18S rDNA Non-concerted evolution Pseudogene Recombination Polymorphism
Document Type期刊论文
Affiliation1.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Natl Engn Res Ctr Facilitated Marine Aquaculture, Zhoushan 316022, Peoples R China
Recommended Citation
GB/T 7714
Yang, Min,Kong, Xiaoyu,Shi, Wei,et al. Remarkable sequence polymorphisms in 18S rDNA of Pleuronichthys cornutus (Pleuronectiformes: Pleuronectidae)[J]. GENE,2018,677:251, 258.
APA Yang, Min,Kong, Xiaoyu,Shi, Wei,Gong, Li,Luo, Hairong,&Wu, Baosheng.(2018).Remarkable sequence polymorphisms in 18S rDNA of Pleuronichthys cornutus (Pleuronectiformes: Pleuronectidae).GENE,677,251.
MLA Yang, Min,et al."Remarkable sequence polymorphisms in 18S rDNA of Pleuronichthys cornutus (Pleuronectiformes: Pleuronectidae)".GENE 677(2018):251.
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