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Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components
Zhang, Yan-Jiao; Lin, Huiyuan; Wang, Pan; Chen, Chang2; Chen, Shiyong1,2
2018
Source PublicationANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
Volume111Issue:11Pages:1985
AbstractVibrio vulnificus, a Gram-negative halophilic bacterium, is an opportunistic human pathogen that is responsible for the majority of seafood-associated deaths worldwide. Lipoproteins are important components of the bacterial cell envelope and have been shown to be involved in a wide variety of cellular processes. Little is known about the localisation or transport mechanism of lipoproteins in V. vulnificus. To assess the localisation of lipoproteins in V. vulnificus, we tested two established techniques for the rapid separation of membrane-associated proteins: detergent extraction with Sarkosyl and outer membrane vesicles (OMVs) preparation. The results showed that Sarkosyl extraction was not useful for the separation of lipoproteins from the different membranes of V. vulnificus. On the other hand, we confirmed that OMVs produced by V. vulnificus contained lipoproteins from the outer but not the inner membrane. Analysis of the OMVs components confirmed the localisation of several well-known lipoproteins to membranes that were different from expected, based on their predicted functions. Using this technique, we found that Asp at position +2 of mature lipoproteins can function as an inner membrane retention signal in V. vulnificus. Interestingly, the Escherichia coli +2 rule does not apply to the V. vulnificus lipoprotein IlpA (G2D) mutant, as a Ser to Asp mutation at position +2 of IlpA did not affect its outer membrane localisation. Furthermore, an IlpA tether-mRFP chimeric lipoprotein and its G2D mutant also behaved like IlpA. Together, these results suggest that the sorting rule of lipoproteins in V. vulnificus might be different from that in E. coli.
DepartmentLMB
Keyword+2 rule Lipoprotein Localisation Sorting Outer membrane vesicles Vibrio vulnificus
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17615
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Qingdao Agr Univ, Sch Life Sci, Shandong Prov Key Lab Appl Mycol, Qingdao 266109, Shandong, Peoples R China
2.Qingdao Agr Univ, Marine Sci & Engn Coll, Shandong Engn Res Ctr Aquat Anim Immune Preparat, Qingdao 266109, Shandong, Peoples R China
3.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yan-Jiao,Lin, Huiyuan,Wang, Pan,et al. Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2018,111(11):1985, 1997.
APA Zhang, Yan-Jiao,Lin, Huiyuan,Wang, Pan,Chen, Chang,&Chen, Shiyong.(2018).Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,111(11),1985.
MLA Zhang, Yan-Jiao,et al."Subcellular localisation of lipoproteins of Vibrio vulnificus by the identification of outer membrane vesicles components".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 111.11(2018):1985.
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