Knowledge Management System Of South China Sea Institute of Oceanology,CAS
| Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis | |
| Huang, Youhua; Huang, Xiaohong; Wang, Shaowen; Yu, Yepin; Ni, Songwei1,2; Qin, Qiwei | |
| 2018 | |
| Source Publication | ARCHIVES OF VIROLOGY
 |
| ISSN | 0304-8608 |
| Volume | 163Issue:11Pages:3023 |
| Abstract | Ranaviruses are nucleoplasmic large DNA viruses that can cause major economic losses in the aquaculture industry and pose a severe threat to global ecological diversity. The available literature demonstrates that classifiable members of the genus Ranavirus enter cells via multiple and complicated routes. Here, we demonstrated the underlying cellular entry mechanism of soft-shelled turtle iridovirus (STIV) using green fluorescence tagged recombinant virus. Treatment with chlorpromazine, sucrose, ethyl-isopropyl amiloride, chloroquine or bafilomycin A1 all significantly decreased STIV infection, suggesting that STIV uses clathrin-mediated endocytosis and macropinocytosis to enter cells via a pH-dependent pathway. Depletion of cellular cholesterol with methyl-beta-cyclodextrin significantly inhibited STIV entry, but neither filipin III nor nystatin did, suggesting that STIV entry was cholesterol dependent but caveola independent. Treatment with dynasore, genistein, ML-7 or cytochalasin D all significantly inhibited STIV infection, indicating that Rac GTPase and myosin II activity were required for the macropinocytosis-like pathway as well as actin polymerization. Our findings suggest that the molecular events involved in STIV entry are not identical to those of other ranavirus isolates. Our results also extend our understanding of the molecular mechanism of iridovirus entry and pathogenesis. |
| Department | LMB |
| Document Type | 期刊论文 |
| Identifier | http://ir.scsio.ac.cn/handle/344004/17616 |
| Collection | 中科院海洋生物资源可持续利用重点实验室 |
| Affiliation | 1.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, 164 West Xingang Rd, Guangzhou 510301, Guangdong, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Huang, Youhua,Huang, Xiaohong,Wang, Shaowen,et al. Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis[J]. ARCHIVES OF VIROLOGY,2018,163(11):3023, 3033. |
| APA | Huang, Youhua,Huang, Xiaohong,Wang, Shaowen,Yu, Yepin,Ni, Songwei,&Qin, Qiwei.(2018).Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis.ARCHIVES OF VIROLOGY,163(11),3023. |
| MLA | Huang, Youhua,et al."Soft-shelled turtle iridovirus enters cells via cholesterol-dependent, clathrin-mediated endocytosis as well as macropinocytosis".ARCHIVES OF VIROLOGY 163.11(2018):3023. |
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| Soft-shelled turtle （4511KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | Application Full Text | |
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