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A novel caspase-6 from sea cucumber Holothuria leucospilota: Molecular characterization, expression analysis and apoptosis detection
Yan, Aifen; Ren, Chunhua1,2; Chen, Ting1,2; Huo, Da1; Jiang, Xiao1; Sun, Hongyan3; Hu, Chaoqun1,2
2018
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume80Pages:232
AbstractIn this study, a novel caspase-6 named HLcaspase-6 was identified from sea cucumber Holothuria leucospilota. The full-length cDNA of HLcaspase-6 is 2195 bp in size, containing a 126 bp 5'-untranslated region (UTR), a 1043 bp 3'-UTR and a 1026 bp open reading frame (ORF) encoding a protein of 341 amino acids with a deduced molecular weight of 38.57 kDa. HLcaspase-6 contains the common signatures of the caspase family, including the conserved pentapeptide motif QACRG, as well as the P20 and P10 domains. In addition, HLcaspase-6 contains a short pro-domain. HLcaspase-6 mRNA is ubiquitously expressed in all tissues examined, with the highest transcript level in the intestine, followed by coelomocytes. In in vitro experiments, the expression of HLcaspase-6 mRNA in coelomocytes was significantly up-regulated by lipopolysaccharides (LPS) or polyriboinosinic-polyribocytidylic acid [poly (I:C)] challenge, suggesting that HLcaspase-6 might play important roles in the innate immune defense of sea cucumber against bacterial and viral infections. Moreover, we further confirmed that overexpression of HLcaspase-6 could induce apoptosis and activate the p53 signal pathway.
DepartmentLMB
KeywordCaspase-6 Holothuria leucospilota Apoptosis Innate immunity p53 signal pathway
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17641
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Foshan Univ, Sch Stomatol & Med, Foshan 528000, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China
3.South China Sea Bioresource Exploitat & Utilizat, Guangzhou, Guangdong, Peoples R China
4.South China Agr Univ, Coll Marine Sci, Guangzhou 510642, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Yan, Aifen,Ren, Chunhua,Chen, Ting,et al. A novel caspase-6 from sea cucumber Holothuria leucospilota: Molecular characterization, expression analysis and apoptosis detection[J]. FISH & SHELLFISH IMMUNOLOGY,2018,80:232, 240.
APA Yan, Aifen.,Ren, Chunhua.,Chen, Ting.,Huo, Da.,Jiang, Xiao.,...&Hu, Chaoqun.(2018).A novel caspase-6 from sea cucumber Holothuria leucospilota: Molecular characterization, expression analysis and apoptosis detection.FISH & SHELLFISH IMMUNOLOGY,80,232.
MLA Yan, Aifen,et al."A novel caspase-6 from sea cucumber Holothuria leucospilota: Molecular characterization, expression analysis and apoptosis detection".FISH & SHELLFISH IMMUNOLOGY 80(2018):232.
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