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Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis
Liu, Yu-Hong; Alimujiang, Adili; Wang, Xiang3; Luo, Shan-Wei; Balamurugan, Srinivasan; Yang, Wei-Dong; Liu, Jie-Sheng; Zhang, Lin2; Li, Hong-Ye
AbstractHaematococcus pluvialis is a main biological resource for the antioxidant astaxanthin production, however, potential modulators and molecular mechanisms underpinning astaxanthin accumulation remain largely obscured. We discovered that provision of ethanol (0.4%) significantly triggered the cellular astaxanthin content up to 3.85% on the 4th day of treatment. Amongst, 95% of the accumulated astaxanthin was esterified, particularly enriched with monoesters. Ultrastructural analysis revealed that ethanol altered cell wall structure and physiological properties. Antioxidant analyses revealed that astaxanthin accumulation offset the ethanol induced oxidative stress. Ethanol treatment reduced carbohydrates while increased lipids and jasmonic acid production. Transcriptomic analysis uncovered that ethanol orchestrated the expression of crucial genes involved in carotenogenesis, e.g. PSY, BKT and CRTR-b were significantly upregulated. Moreover, methyl jasmonic acid synthesis was induced and played a major role in regulating the carotenogenic genes. The findings uncovered the novel viewpoint in the intricate transcriptional regulatory mechanisms of astaxanthin biosynthesis.
KeywordHaematococcus pluvialis Astaxanthin Ethanol induction Jasmonate pathway Transcriptional regulation
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Document Type期刊论文
Affiliation1.Jinan Univ, Coll Life Sci & Technol, Key Lab Eutrophicat & Red Tide Prevent Guangdong, Guangzhou 510632, Guangdong, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
3.Georgetown Univ, Dept Biostat Bioinformat & Biomath, Washington, DC 20057 USA
4.City Univ Hong Kong Kowloon, Sch Energy & Environm, Kowloon, Hong Kong 999077, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yu-Hong,Alimujiang, Adili,Wang, Xiang,et al. Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis[J]. BIORESOURCE TECHNOLOGY,2019,289:121720.
APA Liu, Yu-Hong.,Alimujiang, Adili.,Wang, Xiang.,Luo, Shan-Wei.,Balamurugan, Srinivasan.,...&Li, Hong-Ye.(2019).Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis.BIORESOURCE TECHNOLOGY,289,121720.
MLA Liu, Yu-Hong,et al."Ethanol induced jasmonate pathway promotes astaxanthin hyperaccumulation in Haematococcus pluvialis".BIORESOURCE TECHNOLOGY 289(2019):121720.
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