SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Structure and allosteric coupling of type II antitoxin CopA(SO)
Zhao, Rongjuan1,2; Li, Quan1,2; Zhang, Jingiing1,2; Li, Fudong3; Yao, Jianyun4; Zhang, Jiahai3; Liu, Lin1,2; Wang, Xiaoxue4; Zhang, Xuecheng1,2
2019
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume514Issue:4Pages:1122
AbstractToxin-antitoxin (TA) systems play critical roles in the environment adaptation of bacteria. Allosteric coupling between the N-terminal DNA-binding domain and the C-terminal toxin-binding domain of antitoxins contributes to conditional cooperativity in the functioning of type II TA. Herein, using circular dichroism (CD), nuclear magnetic resonance (NMR), X-ray crystallography, and size exclusion chromatography (SEC), the structure and DNA binding of CopAso, a newly identified type II antitoxin in Shewanella oneidensis, were investigated. Our data show that CopAso is a typical RHH antitoxin with an ordered N-terminal domain and a disordered C-terminal domain, and furthermore indicate that the C terminal domain facilitates DNA binding of the N-terminal domain, which in turn induces the C-terminal domain to fold and associate. (C) 2019 Elsevier Inc. All rights reserved.
DepartmentLMB
KeywordAllosteric coupling Antitoxin CopA(SO) Ribbon-helix-helix motif Shewanella oneidensis
DOI10.1016/j.bbrc.2019.05.049
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17810
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
2.Anhui Prov Engn Technol Res Ctr Microorganisms &, Hefei 230601, Anhui, Peoples R China
3.Anhui Key Lab Modern Biomfg, Hefei 230601, Anhui, Peoples R China
4.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
5.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn, RNAM Ctr Marine Microbio,South China Sea Inst Oce, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Rongjuan,Li, Quan,Zhang, Jingiing,et al. Structure and allosteric coupling of type II antitoxin CopA(SO)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,514(4):1122, 1127.
APA Zhao, Rongjuan.,Li, Quan.,Zhang, Jingiing.,Li, Fudong.,Yao, Jianyun.,...&Zhang, Xuecheng.(2019).Structure and allosteric coupling of type II antitoxin CopA(SO).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,514(4),1122.
MLA Zhao, Rongjuan,et al."Structure and allosteric coupling of type II antitoxin CopA(SO)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 514.4(2019):1122.
Files in This Item:
File Name/Size DocType Version Access License
Structure and allost(992KB)期刊论文出版稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.