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Structure and allosteric coupling of type II antitoxin CopA(SO)
Zhao, Rongjuan1,2; Li, Quan1,2; Zhang, Jingiing1,2; Li, Fudong3; Yao, Jianyun4; Zhang, Jiahai3; Liu, Lin1,2; Wang, Xiaoxue4; Zhang, Xuecheng1,2
AbstractToxin-antitoxin (TA) systems play critical roles in the environment adaptation of bacteria. Allosteric coupling between the N-terminal DNA-binding domain and the C-terminal toxin-binding domain of antitoxins contributes to conditional cooperativity in the functioning of type II TA. Herein, using circular dichroism (CD), nuclear magnetic resonance (NMR), X-ray crystallography, and size exclusion chromatography (SEC), the structure and DNA binding of CopAso, a newly identified type II antitoxin in Shewanella oneidensis, were investigated. Our data show that CopAso is a typical RHH antitoxin with an ordered N-terminal domain and a disordered C-terminal domain, and furthermore indicate that the C terminal domain facilitates DNA binding of the N-terminal domain, which in turn induces the C-terminal domain to fold and associate. (C) 2019 Elsevier Inc. All rights reserved.
KeywordAllosteric coupling Antitoxin CopA(SO) Ribbon-helix-helix motif Shewanella oneidensis
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Document Type期刊论文
Affiliation1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
2.Anhui Prov Engn Technol Res Ctr Microorganisms &, Hefei 230601, Anhui, Peoples R China
3.Anhui Key Lab Modern Biomfg, Hefei 230601, Anhui, Peoples R China
4.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China
5.Chinese Acad Sci, Inst South China Sea Ecol & Environm Engn, RNAM Ctr Marine Microbio,South China Sea Inst Oce, CAS Key Lab Trop Marine Bioresources & Ecol,Guang, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Rongjuan,Li, Quan,Zhang, Jingiing,et al. Structure and allosteric coupling of type II antitoxin CopA(SO)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,514(4):1122, 1127.
APA Zhao, Rongjuan.,Li, Quan.,Zhang, Jingiing.,Li, Fudong.,Yao, Jianyun.,...&Zhang, Xuecheng.(2019).Structure and allosteric coupling of type II antitoxin CopA(SO).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,514(4),1122.
MLA Zhao, Rongjuan,et al."Structure and allosteric coupling of type II antitoxin CopA(SO)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 514.4(2019):1122.
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