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Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata
Wu Xiangwei1,3; Liu Xiande2; Yu Ziniu3
2019
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume37Issue:4Pages:1301
AbstractIncreasingly, exogenous stressors such as pathogen infections, variable water conditions, and pollution are resulting in high mortality of Paphia undulata, deleteriously affecting the quality of clam harvests. The foot is a burrowing organ in clams. Physical damage and constant contact with the external environment cause the foot to be highly sensitive to pathogen invasion and water condition variation. In the present study, the foot tissue transcriptome was analyzed to identify genes involved in immune and stress responses. The P. undulata transcriptome included 5 286 668 078 bp reads generated by Illumina Hiseq 2000 sequencing and were assembled into 1 785 226 contigs by de novo method. The contigs were clustered into 99 339 transcripts and further grouped into 60 201 unigenes. Of them, 22 260 unigenes were successfully annotated using public databases. Twelve genes that were response to immune and stress were identified with abundant expression levels, including heat shock protein 70, cold shock protein, complement C3, cathepsin L, ubiquitin carboxyl-terminal hydrolase L5, and translationally controlled tumor protein. Furthermore, 566 unigenes were found homologous to genes involved in the immune response systems of pathogen discrimination, signal transduction, and immune effector, such as lectins, toll-like receptors, complement pathway, toll-like receptor signaling pathway, heat shock proteins, antioxidant enzymes, lysozymes, and mucins, indicating that P. undulata could have a complete set of innate immune mechanisms. In addition, 4 270 microsatellite markers (SSRs) were identified from 60 201 unigenes, of which trinucleotide repeats were most abundant and 16 SSRs were tested to be polymorphic. The present study provides a new insight into innate immunity and stress response mechanisms in P. undulata.
DepartmentLMB
KeywordPaphia undulata foot tissue transcriptome innate immunity unigene microsatellite
DOI10.1007/s00343-019-8154-5
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17816
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
2.Yunnan Agr Univ, Anim Sci & Technol Coll, Kunming 650201, Yunnan, Peoples R China
3.Jimei Univ, Minist Agr China, Key Lab Hlth Mariculture East China Sea, Fisheries Coll, Xiamen 361021, Fujian, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wu Xiangwei,Liu Xiande,Yu Ziniu. Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(4):1301, 1316.
APA Wu Xiangwei,Liu Xiande,&Yu Ziniu.(2019).Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(4),1301.
MLA Wu Xiangwei,et al."Analysis of novel immune-related genes and microsatellite markers in the transcriptome of Paphia undulata".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.4(2019):1301.
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