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Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway
Xie, Qing; Yang, Zhijie1; Huang, Xuanmei; Zhang, Zikang; Li, Jiangbin; Ju, Jianhua1; Zhang, Hua; Ma, Junying1
2019
Source PublicationJOURNAL OF HEMATOLOGY & ONCOLOGY
ISSN1756-8722
Volume12Pages:60
AbstractBackgroundTriple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer with poor prognosis, and its treatment remains a challenge due to few targeted medicines and high risk of relapse, metastasis, and drug resistance. Thus, more effective drugs and new regimens for the therapy of TNBC are urgently needed. Ilamycins are a kind of cyclic peptides and produced by Streptomyces atratus and Streptomyces islandicus with effective anti-tuberculosis activity. Ilamycin C is a novel compound isolated from the deep South China Sea-derived Streptomyces atratus SCSIO ZH16 and exhibited a strong cytotoxic activity against several cancers including breast cancer cell line MCF7. However, the cytotoxic activity of Ilamycin C to TNBC cells and a detailed antitumor mechanism have not been reported.MethodsCCK-8 assays were used to examine cell viability and cytotoxic activity of Ilamycin C to TNBC, non-TNBC MCF7, and nonmalignant MCF10A cells. EdU assays and flow cytometry were performed to assess cell proliferation and cell apoptosis. Transwell migration and Matrigel invasion assays were utilized to assess the migratory and invading capacity of TNBC cells following the treatment of Ilamycin C. The expressions of proteins were detected by western blot.ResultsIn this study, we found that Ilamycin C has more preferential cytotoxicity in TNBC cells than non-TNBC MCF7 and nonmalignant MCF10A cells. Notably, our studies revealed the mechanism that Ilamycin C can induce Bax/Bcl-2-related caspase-dependent apoptosis and inhibit migration and invasion through MMP2/MMP9/vimentin/fascin in TNBC by suppressing IL-6-induced STAT3 phosphorylation.ConclusionsThis study provides the first evidence that Ilamycin C has significant implications for the potential as a novel IL-6/STAT3 inhibitor for TNBC treatment in the future.
DepartmentLMB
KeywordIlamycin C Triple-negative breast cancer Apoptosis Invasion Migration IL-6 STAT3
DOI10.1186/s13045-019-0744-3
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17827
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Guangdong Med Univ, Dept Clin Biochem, Guangdong Prov Key Lab Med Mol Diagnost, Inst Clin Lab Med, Dongguan 523808, Peoples R China
2.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med,RNAM Ctr Marine, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Xie, Qing,Yang, Zhijie,Huang, Xuanmei,et al. Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway[J]. JOURNAL OF HEMATOLOGY & ONCOLOGY,2019,12:60.
APA Xie, Qing.,Yang, Zhijie.,Huang, Xuanmei.,Zhang, Zikang.,Li, Jiangbin.,...&Ma, Junying.(2019).Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway.JOURNAL OF HEMATOLOGY & ONCOLOGY,12,60.
MLA Xie, Qing,et al."Ilamycin C induces apoptosis and inhibits migration and invasion in triple-negative breast cancer by suppressing IL-6/STAT3 pathway".JOURNAL OF HEMATOLOGY & ONCOLOGY 12(2019):60.
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