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Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae)
Gong, Li1; Luo, Hairong2,3; Shi, Wei2,3; Yang, Min2,3
2019
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
Volume513Issue:3Pages:726
AbstractThe eukaryotic ribosomal DNA (rDNA) cluster consists of multiple copies of three genes (18S, 5.8S, and 28S rDNA) and two internal transcribed spacers (ITS1 and ITS2). Typically, rDNA is thought to be evolved in a strict concerted evolution pattern, in which show little or even no variations within species, while sequence diversity between species or higher taxa. However, in the present study, we reported a high level of intra-individual polymorphism of ITS1-5.8S-ITS2 rDNA in the genome of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae), suggesting it conformed a non-concerted evolutionary pattern. Three divergent type sequences (Type A, B, and C) differing in length, GC content, secondary structure stability and minimum free energy coexisted in the genome of each studied individual. According to the particular features of ribosomal pseudogenes, Type B and Type C sequences were postulated as pseudogenes. Expression analyses gave clear evidence that both the functional gene and pseudogenes were abundantly transcribed, indicating expression is not an applicable criterion for identifying ribosomal pseudogenes. These findings will enrich useful data for the studies on rDNA evolution in flatfishes, as well as provide fresh insight on the transcribed ribosomal pseudogenes and further consideration of the definition of ribosomal pseudogene. (C) 2019 Elsevier Inc. All rights reserved.
DepartmentLMB
KeywordRibosomal RNA Non-concerted evolution Transcribed pseudogene Expression RT-PCR
DOI10.1016/j.bbrc.2019.04.064
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17830
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Zhejiang Ocean Univ, Natl Engn Lab Marine Germplasm Resources Explorat, Zhoushan 316022, Peoples R China
2.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Natl Engn Res Ctr Facilitated Marine Aquaculture, Zhoushan 316022, Peoples R China
3.Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510000, Guangdong, Peoples R China
4.South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510000, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Gong, Li,Luo, Hairong,Shi, Wei,et al. Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae)[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2019,513(3):726, 731.
APA Gong, Li,Luo, Hairong,Shi, Wei,&Yang, Min.(2019).Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae).BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,513(3),726.
MLA Gong, Li,et al."Intra-individual variation and transcribed pseudogenes in the ribosomal ITS1-5.8S-ITS2 rDNA of Paraplagusia japonica (Pleuronectiformes: Cynoglossidae)".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 513.3(2019):726.
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