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Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon
Zeng, Zhenshun; Zhan, Waner1,2; Wang, Weiquan1,2; Wang, Pengxia1; Tang, Kaihao1; Wang, Xiaoxue1,2
2019
Source PublicationFEMS MICROBIOLOGY ECOLOGY
ISSN0168-6496
Volume95Issue:6Pages:fiz065
AbstractBacterial capsular polysaccharides (CPSs) participate in environmental adaptation in diverse bacteria species. However, the role and regulation of CPS production in marine bacteria have remained largely unexplored. We previously reported that both wrinkled and translucent Pseudoalteromonas lipolytica variants with altered polysaccharide production were generated in pellicle biofilm-associated cells. In this study, we observed that translucent variants were generated at a rate of similar to 20% in colony biofilms of P. lipolytica cultured on HSLB agar plates for 12 days. The DNA sequencing results revealed that nearly 90% of these variants had an IS5-like element inserted within the coding or promoter regions of nine genes in the cps operon. In contrast, IS5 insertion into the cps operon was not detected in planktonic cells. Furthermore, we demonstrated that the IS5 insertion event inactivated CPS production, which leads to a translucent colony morphology. The CPS-deficient variants showed an increased ability to form attached biofilms but exhibited reduced resistance to sublethal concentrations of antibiotics. Moreover, deleting the DNA repair gene recA significantly decreased the frequency of occurrence of CPS-deficient variants during biofilm formation. Thus, IS insertion into the cps operon is an important mechanism for the production of genetic variants during biofilm formation of marine bacteria.
DepartmentLMB
Keywordinsertion sequence capsular polysaccharide biofilm Pseudoalteromonas
DOI10.1093/femsec/fiz065
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/17831
Collection中科院海洋生物资源可持续利用重点实验室
Affiliation1.Guangzhou Univ, Sch Environm Sci & Engn, Key Lab Water Qual & Conservat Pearl River Delta, Minist Educ, Guangzhou 510006, Guangdong, Peoples R China
2.Chinese Acad Sci, RNAM Ctr Marine Microbiol, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol,Guangdong, Guangzhou 510301, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zeng, Zhenshun,Zhan, Waner,Wang, Weiquan,et al. Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon[J]. FEMS MICROBIOLOGY ECOLOGY,2019,95(6):fiz065.
APA Zeng, Zhenshun,Zhan, Waner,Wang, Weiquan,Wang, Pengxia,Tang, Kaihao,&Wang, Xiaoxue.(2019).Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon.FEMS MICROBIOLOGY ECOLOGY,95(6),fiz065.
MLA Zeng, Zhenshun,et al."Biofilm formation in Pseudoalteromonas lipolytica is related to IS5-like insertions in the capsular polysaccharide operon".FEMS MICROBIOLOGY ECOLOGY 95.6(2019):fiz065.
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